高通量测序白茅转录组分析及黄酮合成相关基因挖掘  

Transcriptome Analysis of Imperata cylindrica by High-throughput Sequencing and Gene Mining Related to Flavonoid Synthesis

作　　者：李秋玲[1] 郑晓艳[1] 谢伟容[1] 黄建军[1] Li Qiuling;Zheng Xiaoyan;Xie Weirong;Huang Jianjun(College of Pharmacy,Zhangzhou Health Vocational College,Zhangzhou,363000)

机构地区：[1]漳州卫生职业学院药学院,漳州363000

出　　处：《分子植物育种》2024年第14期4574-4582,共9页Molecular Plant Breeding

基　　金：福建省(山区)作物遗传改良与创新利用重点实验室开放课题资助项目(2022SKF04);福建省中青年教师教育科研项目(JAT191425);漳州卫生职业学院科研项目(ZWYZ202319)共同资助。

摘　　要：利用Illumina Hiseq对白茅的地上部分(叶和茎)和地下部分(根茎和根)进行转录组测序及生物信息学分析,挖掘黄酮合成相关基因。结果表明:共获得161775条高质量unigene,N50长度为969 bp,平均长度为145.86 bp,Q20碱基百分比分布在98.19%~98.62%,Q30的碱基百分比分布在94.05%~95.28%。将转录本与GO、KEGG等七大数据库比对,得到其功能注释信息。结果表明,有97922条unigene至少注释到1个数据库,占60.53%;白茅的地上部分对地下部分差异表达unigene有3296个,其中上调表达2574个,下调表达722个。通过对KEGG数据库的代谢通路进行分析,参与白茅黄酮类合成途径相关的有12个酶。白茅参与黄酮类化合物合成的关键基因TMP值,对比关键基因在白茅地上部分及地下部分的表达,发现8个基因在地下部分有较高表达,4个基因在地上部分有较高表达(TMP>15)。本次转录组测序,获得了大量的白茅基因序列,为今后进一步挖掘白茅多种药用活性成分的功能基因、生物合成调控、种质改良等研究提供理论基础。Imperata cylindrica(L.)P.Beauv.to identify genes related to flavonoid synthesis.The results indicated that a total of 161775 high-quality unigenes were obtained,with an N50 length of 969 bp and an average length of 145.86 bp.The percentage of Q20 bases ranged from 98.19%to 98.62%,while the percentage of Q30 bases varied between 94.05%and 95.28%.The transcripts were compared with seven major databases,including GO and KEGG,for functional annotation.It was found that 97922 unigenes were annotated in at least one database,accounting for 60.53%of the total.There were 3296 differentially expressed unigenes between the aboveground and underground parts of Imperata cylindrica,with 2574 upregulated and 722 downregulated.The analysis of metabolic pathways in the KEGG database revealed that 12 enzymes are involved in the flavonoid biosynthesis pathway of Imperata cylindrica.The TMP values of key genes involved in flavonoid biosynthesis were compared between the aboveground and underground parts,revealing that 8 genes were highly expressed in the underground parts,and 4 genes were highly expressed in the aboveground parts(TMP>15).This transcriptome sequencing effort has generated a vast amount of gene sequences for Imperata cylindrica,providing a theoretical basis for further research into the functional genes,biosynthesis regulation,and germplasm improvement for the medicinal active components of Imperata cylindrica.

关 键 词：白茅 转录组分析 差异表达 黄酮 生物信息学 

分 类 号：S567.239[农业科学—中草药栽培]