番茄种质几种病害抗性基因的分子标记检测  

Molecular Marker Detection of Several Disease Resistance Genes in Tomato Germplasm

作　　者：王海娟 魏瑶 刘燕 郑于莉 郭志慧 岳建英 尚春明 赵明敏[1] Wang Haijuan;Wei Yao;Liu Yan;Zheng Yuli;Guo Zhihui;Yue Jianying;Shang Chunming;Zhao Mingmin(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot,010011;Inner Mongolia Baotou Agricultural and Animal Husbandry Research Institute,Baotou,014013;Comprehensive Security and Technology Promotion Center of Ordong Town,Xinghe County,Ulanqab City,Ulanqab City,Ulanqab,012000)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010011 [2]内蒙古包头市农牧业科学研究院,包头014013 [3]乌兰察布市兴和县鄂尔栋镇综合保障和技术推广中心,乌兰察布012000

出　　处：《分子植物育种》2024年第14期4590-4609,共20页Molecular Plant Breeding

基　　金：内蒙古自治区科技重大专项(zdzx2018002);内蒙古农业大学高层次人才科研启动基金项目(NDGCC2016-23)共同资助。

摘　　要：番茄病害在番茄生产中危害非常严重,可导致番茄产量和品质的大幅度降低。目前,抗病番茄种质资源较少。因此,本研究拟对不同番茄的种质材料进行番茄根腐病、番茄斑萎病和番茄黄化曲叶病毒病的抗性基因进行分子标记检测。根据已报道的抗性基因的分子标记引物,对番茄种质材料进行PCR扩增,获得抗性基因的特异性片段,在抗根腐病的分子标记中筛选出83份番茄种质为纯合体抗病体;在抗斑萎病的分子标记中筛选出4份番茄种质为纯合体抗病体;在抗番茄黄化曲叶病毒病分子标记中,筛选出39份番茄种质纯合抗病体。研究结果可为番茄抗病育种的快速鉴定提供理论依据。Tomato diseases are very harmful in tomato production,which can lead to a substantial decrease in tomato yield and quality.At present,there are few germplasm resources of disease-resistant tomato.Therefore,this study intends to detect the resistance genes of tomato root rot,tomato spot wilt and tomato yellow leaf curl virus on different tomato germplasm materials for molecular marker detection.According to the molecular marker primers of the reported resistance genes,PCR was performed on tomato germplasm materials to obtain specific fragments of the resistance genes.83 tomato germplasms were screened out as homozygous from the molecular markers for root rot resistance.Resistant to pathogens;4 tomato germplasms homozygous resistance were screened out of the molecular markers for resistance to tomato spot wilt virus disease;39 tomato germplasms homozygous resistance were screened out of the molecular markers for resistance to tomato yellow leaf curl virus disease.The research results can provide a theoretical basis for the rapid identification of tomato disease resistance breeding.

关 键 词：番茄病害 抗性基因 分子标记 

分 类 号：S641.2[农业科学—蔬菜学]