赤苍藤RAPD-PCR体系的建立与应用  

Establishment and Application of RAPD-PCR System for Erythropalum scandens

作　　者：赵昊天 祝建波[1] 杨有兴 谭骏 文国荣 王冬梅 Zhao Haotian;Zhu Jianbo;Yang Youxing;Tan Jun;Wen Guorong;Wang Dongmei(College of Life Sciences,Shihezi University,Shihezi,832003;Nanning Institute of Agricultural Sciences,Nanning,530021)

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]广西南宁市农业科学研究所,南宁530021

出　　处：《分子植物育种》2024年第14期4621-4629,共9页Molecular Plant Breeding

基　　金：广西壮族自治区科技厅重点研发计划项目(桂科AB19245042);南宁市农业科学研究所重点科技专项(南农科项2020-3)共同资助。

摘　　要：为揭示赤苍藤的遗传变异情况,本研究采用RAPD-PCR技术对来自18个地区的野生赤苍藤品种进行了遗传多样性分析。通过正交试验和单因素试验确定RAPD-PCR扩增体系的总体积为25.0μL,其中:引物2.0μL;DNA 2.0μL;Master Mix 12.5μL。使用优化后的PCR扩增体系筛选出13条多态性好重复性高的引物,共扩增位点95,多态性位点77,多态百分比为80.55%,遗传相似性系数在0.55~0.86之间,遗传距离范围为0.12~0.60,当遗传相似性系数为0.71时可分为5大类,表明赤苍藤遗传多样性较为丰富且种群内存在较大差异,为优异品种的筛选及开发提供理论基础。To reveal the genetic variation of the Erythropalum scandens.In this study,the RAPD-PCR technology was used to analyze the genetic diversity of wild Erythropalum scandens varieties from 18 regions.Through orthogonal experiment and single factor experiment,the total volume of the RAPD-PCR amplification system is finally determined to be 25.0μL,including 2.0μL of primer;2.0μL of DNA;12.5μL of 2×Taq Master Mix.The optimized PCR amplification system was used to screen out 13 primers with good polymorphism and high reproducibility,with a total of 95 sites for amplification and 77 sites for polymorphism.When the genetic similarity coefficient is 0.71,it can be divided into 5 categories,which indicates that the genetic diversity of is Erythropalum scandens richer and there are large differences in the population.This research provides a theoretical basis for the selection and development of excellent varieties.

关 键 词：赤苍藤 RAPD 遗传多样性 

分 类 号：S567[农业科学—中草药栽培]