利用R语言的AMMI模型分析粳型不育系及其组合产量配合力  

Analyzing the Yield Combining Ability of Japonica Sterile Lines and Their Combinations Using the R Language's AMMI Model

作　　者：丁正权 来乐春 王士磊[1] 潘月云 黄海祥[1] Ding Zhengquan;Lai Lechun;Wang Shilei;Pan Yueyun;Huang Haixiang(Jiaxing Academy of Agricultural Sciences,Jiaxing,314016)

机构地区：[1]嘉兴市农业科学研究院,嘉兴314016

出　　处：《分子植物育种》2024年第14期4648-4656,共9页Molecular Plant Breeding

基　　金：嘉兴市科技计划项目(2020AZ10003,20210801,2021AZ10012);绿色高质量水稻育种创新团队项目(20210801)共同资助。

摘　　要：配合力分析为合理利用杂交水稻亲本、选择强优势杂交组合提供依据。为了研究产量性状的配合力,以近年育成的5个粳型不育系和8个恢复系为亲本,利用R语言agricolae包的AMMI模型对5×8不完全双列杂交设计的40个组合产量性状进行配合力分析。AMMI模型的方差分析结果表明:不育系一般配合力达到极显著(P<0.01),恢复系一般配合力和组合特殊配合力达到显著水平(P<0.05)。双亲中以不育系3684A、3686A和3768A、恢复系GR56、GR27和JR352等一般配合力较高。AMMI模型将组合特殊配合力效应平方和分解为两个显著的主成分(PC1,PC2),分别解释了互作效应平方和的58.5%和33.6%。特殊配合力的主成分分析结果表明:3684A×G3828、3768A×G3828、3686A×GR56、31A×GR27、3768A×JR352和3768A×GR184等组合特殊配合力分值最大,与传统配合力分析方法的结果大小相近,说明用亲本PC1和PC2乘积和能很好地度量组合的特殊配合力。此外,在AMMI1双标图中可以看出亲本之间产量水平的变异和互作效应的差异。在AMMI2双标图中,根据不育系和恢复系互作效应PC1与PC2向量长度与方向,可将杂交组合的互作效应情况进行区分。只有当双亲特殊配合力效应分值向量长度均比较大、向量方向夹角较小时才可能获得正的强互作效应。The combing ability analysis provide basis for rational utilization of hybrid rice parents and selection of superior combinations.To study the combing ability of yield characteristics,five new japonica male steile lines and eight restorer lines were slected to bred fourty hybrid combinations based on 5×8 incomplete dialle cross design by AMMI model of agricolae package on R language.ANOVA analysis by AMMI model showed that general combining ability(GCA)of male sterile lines reached the extremely significant difference level(P<0.01),and GCA of restorer lines and special combining ability(SCA)of hybrid combinations were significant(P<0.05).3684A,3686A and 31A of sterile lines,GR56,GR27 and JR352 of restorer lines had greater GCA variance.The AMMI model extracted two significant principal components,PC1 and PC2,from SCA,which accounted 58.5%and 33.6%variance of the interaction sum of squares,respectively.The SCA effects were estimated by using principal components analysis and classic way.The results of the two methods had only smaller difference which showed that the combinations such as 3684A×G3828,3768A×G3828,3686A×GR56,31A×GR27,3768A×JR352 and 3768A×GR184 had higher SCA effects.Therefore,the sum of products at PC1and PC2 axis of parents can well present SCA effects of hybrid combinations.Furthermore,the parents and their interactions were really differences on yield effect in the AMMI1 biplot.According to the length and direction of PC1 and PC2 vectors on AMMI2 biplot,the interaction effect of sterile line and restorer line can be assessed.The positive strong interaction effect can be obtained only when the length of the score vector of the special combining ability effect of both parents is relatively large and the angle of the vector direction is relatively small.

关 键 词：R语言 AMMI模型 粳型不育系 一般配合力 特殊配合力 

分 类 号：S511[农业科学—作物学]