Correlation analysis of human papillomavirus E6/E7 mRNA detection with diagnosis,prognosis and recurrence risk in patients with cervical epithelioma  

作　　者：Ling-Li Zhang Ming-Yan Du Xin Du Jie Duan Dong-Mei Yao Jing Jing Chun Feng Lin Song 

机构地区：[1]Department of Gynaecology,Maternal and Child Health Hospital of Hubei Province,Wuhan 430075,Hubei Province,China [2]Department of Gynaecology,China Resources WISCO General Hospital,Wuhan 430080,Hubei Province,China [3]Department of Surgery,Maternal and Child Health Hospital of Hubei Province,Wuhan 430075,Hubei Province,China

出　　处：《World Journal of Clinical Cases》2024年第20期4146-4153,共8页世界临床病例杂志

基　　金：Scientific Research Project of Hubei Provincial Health Commission,No.WJ2021M189。

摘　　要：BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and diagnosis.METHODS One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected.These patients were graded CIN based on colposcopy and cervical pathology.The positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all groups.Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr.The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.RESULTS The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,respectively.Sensitivity and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was 0.789.Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.

关 键 词：Cervical epithelial neoplasia Human papilloma change with virus E6/E7 mRNA Cervical cancer Correlation analysis 

分 类 号：R737.33[医药卫生—肿瘤]