Diagnosis and treatment of refractory infectious diseases using nanopore sequencing technology:Three case reports  

作　　者：Qing-Mei Deng Jian Zhang Yi-Yong Zhang Min Jia Du-Shan Ding Yu-Qin Fang Hong-Zhi Wang Hong-Cang Gu 

机构地区：[1]Science Island Branch,Graduate School of University of Science and Technology of China,Hefei 230031,Anhui Province,China [2]Institute of Health and Medical Technology,Hefei Institutes of Physical Science,Chinese Academy of Science,Hefei 230031,Anhui Province,China [3]Medical Pathology Center,Hefei Cancer Hospital,Chinese Academy of Sciences,Hefei 230031,Anhui Province,China

出　　处：《World Journal of Clinical Cases》2024年第22期5208-5216,共9页世界临床病例杂志

基　　金：Supported by Research and Development Funding for Medical and Health Institutions,No.2021YL007.

摘　　要：BACKGROUND Infectious diseases are still one of the greatest threats to human health,and the etiology of 20%of cases of clinical fever is unknown;therefore,rapid identification of pathogens is highly important.Traditional culture methods are only able to detect a limited number of pathogens and are time-consuming;serologic detection has window periods,false-positive and false-negative problems;and nucleic acid molecular detection methods can detect several known pathogens only once.Three-generation nanopore sequencing technology provides new options for identifying pathogens.CASE SUMMARY Case 1:The patient was admitted to the hospital with abdominal pain for three days and cessation of defecation for five days,accompanied by cough and sputum.Nanopore sequencing of the drainage fluid revealed the presence of orallike bacteria,leading to a clinical diagnosis of bronchopleural fistula.Cefoperazone sodium sulbactam treatment was effective.Case 2:The patient was admitted to the hospital with fever and headache,and CT revealed lung inflammation.Antibiotic treatment for Streptococcus pneumoniae,identified through nanopore sequencing of cerebrospinal fluid,was effective.Case 3:The patient was admitted to our hospital with intermittent fever and an enlarged neck mass that had persisted for more than six months.Despite antibacterial treatment,her symptoms worsened.The nanopore sequencing results indicate that voriconazole treatment is effective for Aspergillus brookii.The patient was diagnosed with mixed cell type classical Hodgkin's lymphoma with infection.CONCLUSION Three-generation nanopore sequencing technology allows for rapid and accurate detection of pathogens in human infectious diseases.

关 键 词：Nanopore sequencing technology Third-generation sequencing technology INFECTION PATHOGEN Case report 

分 类 号：R51[医药卫生—内科学] R318[医药卫生—临床医学]