艾绒的化学成分研究  

Chemical constituents from moxa floss

作　　者：雷青会 靳景惠 李东阳 魏文君[1,2] 陈随清 郭涛 LEI Qinghui;JIN Jinghui;LI Dongyang;WEI Wenjun;CHEN Suiqing;GUO Tao(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Engineering Research Center of Medicinal and Edible Chinese Medicine Technology,Zhengzhou 450046,China;Engineering Technology Research Center of TCM Great Health Industry,Henan University of Chinese Medicine,Zhengzhou 450046,China;Zhang Zhongjing Inherited and Innovative Center of Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省药食同源中药技术工程研究中心,河南郑州450046 [3]河南省中医药大健康产业工程技术研究中心,河南郑州450046 [4]河南中医药大学张仲景传承创新中心,河南郑州450046

出　　处：《中草药》2024年第12期3949-3955,共7页Chinese Traditional and Herbal Drugs

基　　金：河南省高层次人才国际化培养项目(豫科[2021]72);河南省重大科技专项(221100310400)。

摘　　要：目的对艾绒的化学成分进行研究。方法采用硅胶、ODS、MCI、Sephadex LH-20和prep HPLC等现代色谱技术对其化学成分进行分离纯化,利用MS、NMR等现代波谱技术鉴定单体化合物结构;采用脂多糖(lipopolysaccharide,LPS)诱导的小鼠巨噬细胞RAW264.7炎症模型,开展新化合物的抗炎活性评价。结果从艾绒醋酸乙酯提取物中分离得到13个化合物,分别鉴定为(4S,5S)-5-[(R)-5'-hydroxy-2'-methyl-3'-oxocyclopent-1'-en-1'-yl]-3-methylene-4-(3-oxobutyl)dihydrofuran-2(3H)-one(1)、iso-seco-tanapartholide(2)、3-methoxy-tanapartholide(3)、3-acetyl-iso-seco-tanapartholide(4)、甲基条叶蓟素(5)、圣草酚(6)、泽兰黄素(7)、半齿泽兰素(8)、茵陈色原酮(9)、矢车菊黄素(10)、柚皮素(11)、高圣草酚(12)、异泽兰黄素(13)。化合物1能够抑制LPS诱导的RAW264.7细胞中NO的生成量,半数抑制浓度(median inhibition concentration,IC_(50))值为(31.15±2.30)μmol/L。结论化合物1为新化合物,并命名为1R-长叶艾菊内酯,化合物4为首次从菊科植物中分离得到;化合物1具有一定的抗炎活性。Objective To investigate the chemical constituents of moxa floss.Methods The chemical constituents were isolated and purified by silica gel,ODS,MCI,Sephadex LH-20 and prep HPLC,and their structures were elucidated using MS and NMR.Meanwhile,the Griess method was used to evaluate the anti-inflammatory activity by testing the inflammatory factor release model induced by lipopolysaccharide(LPS)in mouse macrophage RAW264.7 cells.Results A total of 13 compounds were isolated from the ethyl acetate extract of moxa floss and identified as(4S,5S)-5-((R)-5'-hydroxy-2'-methyl-3'-oxocyclopent-1'-en-1'-yl)-3-methylene-4-(3-oxobutyl)dihydrofuran-2(3H)-one(1),iso-seco-tanapartholide(2),3-methoxy-tanapartho-lide(3),3-acetyl-iso-seco-tanapartholide(4),cirsilineol(5),eriodictyol(6),nepetin(7),eupatorin(8),capillarisin(9),centaureidin(10),naringenin(11),homoeriodictyol(12),and eupatilin(13).In vitro anti-inflammatory assays showed that compound 1 inhibited the release of NO in RAW264.7 cells induced by LPS with IC_(50) value of 31.15±2.30μmol/L.Conclusion Compound 1 was a new compound,which was identified as 1R-tanaphillin,and compound 4 was isolated from Compositae for the first time.Compound 1 had certain antiinflammatory activity.

关 键 词：艾 艾绒 倍半萜类 黄酮类 抗炎 1R-长叶艾菊内酯 3-acetyl-iso-seco-tanapartholide 圣草酚 泽兰黄素 柚皮素 高圣草酚 

分 类 号：R284.1[医药卫生—中药学]