马鞭草中1个新生物碱成分  

A new alkaloid isolated from Verbena officinalis

作　　者：王加文[1,2] 李洁 刘建群 彭财英[1] 舒积成[1] WANG Jiawen;LI Jie;LIU Jianqun;PENG Caiying;SHU Jicheng(Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi IndustryPolytechnic College,Nanchang 330095,China)

机构地区：[1]江西中医药大学,江西南昌330004 [2]江西工业职业技术学院,江西南昌330095

出　　处：《中草药》2024年第11期3588-3592,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(82160660);江西省自然科学基金项目(20212ACB206006);江西中医药大学“1050”工程人才项目(5142001004);江西中医药大学创新团队(CXTD22015)。

摘　　要：目的研究马鞭草Verbena officinalis醋酸乙酯抗炎活性部位的化学成分。方法运用多种色谱技术和波谱手段进行分离、纯化和鉴定化合物,通过抑制一氧化氮(NO)生成实验评价新化合物的抗炎活性。结果从马鞭草醋酸乙酯部位中分离得到8个生物碱类成分,分别为马鞭草碱A(verboffidine A,1)、5-羟基-2-吡啶甲酯(2)、6-羟甲基-3-羟基吡啶(3)、烟酸(4)、尿嘧啶苷(5)、腺苷(6)、苯乙胺(7)和吲哚-3-乙醇(8)。体外抗炎实验显示,化合物1具有抑制NO分泌作用,半数抑制浓度(median inhibition concentration,IC_(50))为38.5μmol/L。结论化合物1为新化合物,命名为马鞭草碱A(verboffidine A),并且具有潜在的抗炎活性;化合物2、3、7和8为首次从马鞭草中分离得到。Objective To investigatethe chemical components of Verbena officinalisof the elthyl acetate fraction with anti-inflammatory activity.Methods The compounds were isolated,purifiedand identified by various chromatographic techniques and spectralmethods.The anti-inflammatory activity of the new compound was evaluated by inhibiting the production of nitric oxide(NO).Results A total of eight alkaloids were isolated from the elthyl acetate fraction of V.officinalis,and their structures were identifiedas follows:verboffidine A(1),methyl-5-hydroxy-2-pyridinecarboxylate(2),6-hydroxymethyl-3-pyridinol(3),nicotinic acid(4),uridine(5),adenosine(6),phenethylamine(7)and 2-(1H-indol-3-yl)ethanol(8).In vitroanti-inflammatory activity experiments showed that compound 1could inhibit NO secretionwith a median inhibition concentration(IC_(50))of 38.5μmol/L.Conclusion Compound 1was a new compound,named verboffidine A,and hadpotential anti-inflammatory activity.Compounds 2,3,7and8were isolated for the first time from V.officinalis.

关 键 词：马鞭草 生物碱 马鞭草碱A 抗炎 5-羟基-2-吡啶甲酯 6-羟甲基-3-羟基吡啶 苯乙胺 吲哚-3-乙醇 

分 类 号：R284.1[医药卫生—中药学]