棒柄杯伞的化学成分研究  

Chemical constituents from Clitocybe clavipes

作　　者：雷启军 逯永满[1] 马国需 孙照翠 许旭东 石磊岭 吕杰[1] LEI Qijun;LU Yongman;MA Guoxu;SUN Zhaocui;XU Xudong;SHI Leiling;LYU Jie(Xinjiang University,Urumqi 830046,China;Xinjiang Institute of Traditional Chinese Medicine and Ethnic Medicine,Urumqi 830002,China;Institute of Medicinal Plant Development,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100193,China)

机构地区：[1]新疆大学,新疆乌鲁木齐830046 [2]新疆维吾尔自治区中药民族药研究所,新疆乌鲁木齐830002 [3]中国医学科学院北京协和医院药用植物研究所,北京100193

出　　处：《中草药》2024年第11期3602-3608,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(8216130391)。

摘　　要：目的研究担子菌棒柄杯伞Clitocybe clavipes的化学成分。方法采用硅胶、Sephadex LH-20柱色谱和半制备液相等色谱学技术进行分离纯化,通过现代波谱学技术对化合物的结构进行表征和解析。同时采用MTT法测试化合物的体外细胞毒活性。结果从棒柄杯伞95%乙醇提取物中分离鉴定了10个化合物,分别为(1aR,11R,11aR,Z)-9-(aminomethyl)-7,10-dihydroxy-5-methyl-3,6,11,11a-tetrahydro-2H-11,1a-(epoxymethano)benzo[4,5]cyclodeca[1,2-b]oxiren-13-one(1)、clavilactone C(2)、clavilactone D(3)、clavilactone F(4)、clavilactone G(5)、clavilactone J(6)、clavilactone K(7)、arnebinol A(8)、clavipyrrine A(9)、cyclo(Trp-Ala)(10)。化合物1~10对人胃癌HGC-27细胞具有不同程度的增殖抑制作用,其中化合物1、3、8、9的半数抑制浓度(median inhibition concentration,IC_(50))分别为13.57、3.62、7.31、11.51μmol/L。结论化合物1为新化合物,命名为棒柄杯伞内酯Q。化合物10为首次从担子菌棒柄杯伞中分离得到;化合物1、3、8、9具有较为显著的细胞毒活性。Objective To investigate the chemical constituents of BasidiomyceteClitocybe clavipes.Methods Chromatographic techniques including silica gel,Sephadex LH-20 column chromatography and semi-preparative liquid chromatography were employed to isolate and purify the compounds.Their structures were characterized and interpreted by modern wave spectroscopy techniques.The in vitrocytotoxic activity of compounds was determined by MTT assay.Results A total of ten compounds were isolated from the 95%EtOH extract of C.clavipes,which were identified as(1aR,11R,11aR,Z)-9-(aminomethyl)-7,10-dihydroxy-5-methyl-3,6,11,11a-tetrahydro-2H-11,1a-(epoxymethano)benzo[4,5]cyclodeca[1,2-b]oxiren-13-one(1),clavilactone C(2),clavilactone D(3),clavilactone F(4),clavilactone G(5),clavilactone J(6),clavilactone K(7),arnebinol A(8),clavipyrrine A(9)and cyclo(Trp-Ala)(10).The results of cytotoxicity experiments showed that compounds 1-10had different degrees of inhibitory effects on the proliferation of human gastric cancer cells HGC-27,and the median inhibition concentration(IC_(50))values of compounds 1,3,8,and 9were13.57,3.62,7.31 and 11.51μmol/L,respectively.Conclusion Compound 1is a new compound named clavilactone Q,and compound 10is isolated for the first time from BasidiomyceteC.clavipes.Compounds 1,3,8,and 9had significant cytotoxicity.

关 键 词：棒柄杯伞 混源萜 棒柄杯伞内酯Q 细胞毒活性 clavilactone C arnebinol A 

分 类 号：R284.1[医药卫生—中药学]