经典名方泻黄散标准煎液化学成分的UPLC-Q-TOF-MS分析  

UPLC-Q-TOF-MS Analysis on Chemical Constituents of Classical Prescription Xiehuang San Standard Decoction

作　　者：肖婉 王思琦 李家政 富雪丹 鞠建明[2,3] 康安[1] 姚卫峰[1] 江海浪 XIAO Wan;WANG Siqi;LI Jiazheng;FU Xuedan;JU Jianming;KANG An;YAO Weifeng;JIANG Hailang(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Engineering Research Center of Chinese Medicine Preparation,Jiangsu Province Academy of Traditional Chinese Medicine,Nanjing 210028,China;Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中医药研究院中药制剂工程研究中心,江苏南京210028 [3]南京中医药大学附属中西医结合医院,江苏南京210028

出　　处：《南京中医药大学学报》2024年第6期569-581,共13页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省重点研发计划(社会发展)项目(BE2019767);南京中医药大学自然科学基金(XZR2020021)。

摘　　要：目的采用UPLC-Q-TOF-MS技术解析经典名方泻黄散标准煎液的化学成分表征,同时进行化学成分分类,对代表性成分进行分析。方法采用Acquity HSS T_(3)(2.1 mm×100 mm,1.8μm)色谱柱,0.1%甲酸水-0.1%甲酸乙腈为流动相进行梯度洗脱,流速0.4 mL·min^(-1),柱温40℃。在正、负离子模式下采集泻黄散的质谱数据。通过Masslynx 4.1软件与对照品比对、质谱数据分析以及参考相关文献对泻黄散的化学成分进行分析和鉴定。结果从泻黄散中共分析和鉴定出107个化合物,包括黄酮类化合物45个,三萜类27个,单萜类11个,苯丙素类10个,色原酮6个,生物碱类5个以及其他类3个。结论研究结果为泻黄散的进一步药效物质基础研究及质量控制方法的建立提供了实验依据。OBJECTIVE To analyze the chemical constituents from classical prescription Xiehuang San(XHS)standard decoction by UPLC-Q-TOF-MS technology,and classify the chemical composition and analyze the representative components.METHODS Acquity HSS T_(3) column(2.1 mm×100 mm,1.8μm)was used as the chromatographic column,with 0.1% formic acid solution-0.1% formic acid acetonitrile as the mobile phase for gradient elution.The volume flow rate was 0.4 mL·min^(-1) and the column temperature was 40℃.Mass spectrometry data of XHS were collected in positive and negative ion modes.The chemical constituents from classical prescription XHS were analyzed and identified by Masslynx 4.1 software comparison with reference materials,mass spectrometry data analysis and reference to relevant literature.RESULTS A total of 107 compounds were analyzed and identified from XHS,including 45 flavonoids,27 triterpenoids,11 monoterpenoids,10 phenylpropanoids,6 chromogenic ketones,5 alkaloids and 3 other other compounds.CONCLUSION The study provides an experimental basis for the further research on the substance basis and quality control of XHS.

关 键 词：经典名方 泻黄散标准煎液 UPLC-Q-TOF-MS 化学成分 

分 类 号：R284[医药卫生—中药学]