基于气质联用技术的黄芩素防治放射性肠损伤小鼠血清代谢组学分析  

Serum Metabolomics Analysis of Baicalein for Prevention and Treatment of Radiation-Induced Intestinal Damage in Mice Based on GC-MS

作　　者：朱业锦 王心平 王纤易 何骏 张兴德[3] 姜超[2] ZHU Yejin;WANG Xinping;WANG Qianyi;HE Jun;ZHANG Xingde;JIANG Chao(School of Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China;The Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangsu Province Hospital of Chinese Medicine,Nanjing 210029,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学医学院,江苏南京210023 [2]南京中医药大学附属医院/江苏省中医院,江苏南京210029 [3]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2024年第7期702-709,共8页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82304595,82304854);江苏省自然科学基金青年基金项目(BK20220465);国家卫生健康委员会重点实验室开放课题(JSHD2022039);江苏省中医药科技发展计划项目(YB201916)。

摘　　要：目的通过基于气质联用(GC-MS)技术的代谢组学方法,考察黄芩素干预下放射性肠损伤小鼠血清代谢产物变化,以及黄芩素参与放射性肠损伤防治过程中,内源性生物小分子的变化特征,探讨黄芩素潜在的调控机制。方法建立小鼠放射性肠损伤模型,并随机分为正常对照组、模型组、低剂量黄芩素组和高剂量黄芩素组。黄芩素以灌胃方式给予,采用GC-MS技术,对各组小鼠血清样本进行分析,通过偏最小二乘法-判别分析(PLS-DA)筛选差异性代谢物,利用MetaboAnalyst分析潜在的代谢通路。结果小鼠肠组织病理切片显示,黄芩素高剂量组和低剂量组对放射性肠损伤有一定的防护作用。代谢组学分析表明,空白组、模型组、低剂量和高剂量黄芩素给药组代谢谱存在显著差异。黄芩素灌胃给药后,放射性肠损伤小鼠的内源性代谢物有正常化趋势。研究共筛选出11个潜在代谢标志物,以及与此相关的5条代谢通路,其中糖代谢、谷胱甘肽通路、氨代谢相关通路尤为显著。结论黄芩素对放射性肠损伤有一定的防治作用。黄芩素参与糖代谢和谷胱甘肽代谢,改善放射导致的内源性物质紊乱是其潜在作用机制。OBJECTIVE To investigate the changes in serum metabolites of mice with radiation-induced intestinal injury under the intervention of baicalein and the changing characteristics of endogenous biological small molecules during the process of baicalein's participation in the prevention and treatment of radiation-induced intestinal injury through the metabolomics method based on GC-MS technology,in order to explore the potential regulatory mechanism of baicalein.METHODS A mouse radioactive intestinal injury model was established and randomly divided into normal control group,model group,low-dose baicalein group and high-dose baicalein group.Baicalein was administered by gavage.Gas chromatography-mass spectrometry(GC-MS)technology was used to analyze the serum samples of mice in each group,and differential metabolites were screened through partial least squares-discriminant analysis(PLS-DA).Potential metabolic pathways were analyzed with MetaboAnalyst.RESULTS The pathological sections of mouse intestinal tissue showed that the high-dose and low-dose baicalein groups had a certain protective effect on radiation-induced intestinal damage.Metabolomic analysis showed that there were significant differences in the metabolic profiles of the blank control group,model group,low-dose and high-dose baicalein administration groups.After intragastric administration of baicalein,the endogenous metabolites in mice with radiation intestinal injury tended to normalize.The study screened out a total of 11 potential metabolic markers and 5 related metabolic pathways,among which pathways related to glucose metabolism,glutathione pathway,and ammonia metabolism were particularly significant.CONCLUSION Baicalein has a certain preventive and therapeutic effect on radiation-induced intestinal injury;baicalein participates in glucose metabolism and glutathione metabolism,and improving the endogenous substance disorder caused by radiation is its potential mechanism of action.

关 键 词：放射性肠损伤 黄芩素 气质联用 代谢组学 代谢网络分析 

分 类 号：R285.5[医药卫生—中药学]