鹅星状病毒与新型鸭呼肠孤病毒双重RT-PCR检测方法的建立及应用  

Establishment and application of double RT-PCR detection method for goose astrovirus and novel duck reovirus

作　　者：张景辉 武景琦 房立春 刘涛[3] 吴家强[1] 于可响[3] 徐怀英[3] 吕俊峰 艾武[3] 郭慧君[2] 邱建华[2] 亓丽红[1] 宋玲玲[3] ZHANG Jinghui;WU Jingqi;FANG Lichun;LIU Tao;WU Jiaqiang;YU Kexiang;XU Huaiying;LüJunfeng;AI Wu;GUO Huijun;QIU Jianhua;QI Lihong;SONG Lingling(Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250131,China;College of Animal Science and Technology,Shandong Agricultural University,Jinan 250131,China;Poultry Research Institute,Shandong Academy of Agricultural Sciences,Taian 271017,China)

机构地区：[1]山东省农业科学院畜牧兽医研究所,山东济南250131 [2]山东农业大学动物科技学院,山东济南250131 [3]山东省农业科学院家禽研究所,山东泰安271017

出　　处：《中国兽医科学》2024年第6期749-756,共8页Chinese Veterinary Science

基　　金：中央引导地方科技发展资金项目(YDZX2023009);山东省重点研发计划(竞争性创新平台)项目(2022CXPT022);山东省科技型中小企业创新能力提升工程项目(2022TSGC2342)。

摘　　要：根据鹅星状病毒(GAstV)ORF1b基因与鹅源新型鸭呼肠孤病毒(NDRV)σC基因的保守序列设计特异性引物,建立GAstV与NDRV双重RT-PCR检测方法,该方法具有较高的特异性,在903 bp(GAstV)和226 bp(NDRV)可检测到特异性条带,最低灵敏度为1.0×10^(3)copies/μL。在鲁中、鲁西北及鲁南地区不同规模的养鹅场中随机采取320份样本,GAstV的阳性率为21.56%(69/320),NDRV的阳性率为3.44%(11/320),混合感染阳性率为2.81%(9/320),单一RT-PCR结果与双重RT-PCR方法一致。该方法的建立为快速诊断GAstV和NDRV提供了可靠的手段。In this study,specific primers were designed based on the conserved sequence of goose astrovirus(GAst V)ORF1b gene and novel duck reovirus(NDRV)σC gene,and a dual RT-PCR method of GAst V and NDRV was established.This method has high specificity to amplify fragments of 903 bp(GAst V)and 226bp(NDRV).The minimum sensitivity of this method was 1.0×10^(3)copies/μL.In 320 samples randomly taken from goose farms of different scales in the central,northwestern,and southern regions of Shandong,the positive rate of GAst V was 21.56%(69/320),the positive rate of NDRV was 3.44%(11/320),and the positive rate of mixed infection was 2.81%(9/320).The results of the single RT-PCR were consistent with the dual method.This provides a reliable basis for the rapid diagnosis of GAst V and NDRV.

关 键 词：鹅星状病毒 鹅源新型鸭呼肠孤病毒 双重RT-PCR 

分 类 号：S852.659.6[农业科学—基础兽医学]