冰晶调控剂对活疫苗半成品中伪狂犬病病毒效价的影响  

Effect of ice crystal regulator on the titer of pseudorabies virus in semi-manufactured live vaccine

作　　者：赵艳红[1,2,3] 孟繁荣[1] 吕芳 王军宁 左晓昕[2,3] 揭鸿英 卢宇 鲍恩东 ZHAO Yanhong;MENG Fanrong;Lüfang;WANG Junning;ZUO Xiaoxin;JIE Hongying;LU Yu;BAO Endong(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Institute of Veterinary Immunology&Engineering,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Guotai(Taizhou)Center of Technology Innovation for Veterinary Biologicals,Taizhou 225300,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]江苏省农业科学院动物免疫工程研究所,江苏南京210014 [3]兽用生物制品(泰州)国泰技术创新中心,江苏东泰州225300

出　　处：《中国兽医科学》2024年第6期757-765,共9页Chinese Veterinary Science

基　　金：江苏省农业科技自主创新资金项目(CX(22)2016)。

摘　　要：为了解决病毒活疫苗半成品经-20℃保存后猪伪狂犬病病毒效价降低显著的问题,在伪狂犬病病毒悬液中分别添加4类不同浓度的冰晶调控剂后,经5次冻融循环后检测冻融前后病毒效价损失。结果显示,0.25 mol/L AMG、0.14 mol/L Suc、0.45 mmol/L PVP、0.40 mol/L Lac组的病毒效价损失分别为0.20、0.26、0.25、0.31 lg TCID_(50)/m L;将保护效果最好的0.25 mol/L AMG与伪狂犬病病毒悬液混合,制备成活疫苗半成品在-20℃条件下保存60 d后,其病毒效价损失小于0.6 lg TCID_(50)/m L;安全性评价结果显示,0.25 mol/L AMG对小鼠、细胞无明显毒性。结果表明:病毒悬液中添加0.25 mol/L AMG经冻融循环后制备的活疫苗半成品猪伪狂犬病病毒效价损失小,且对小鼠和细胞均具有安全性,这为病毒活疫苗生产的冻干工艺优化提供了重要的理论依据。In order to solve the problem that the titer of porcine pseudorabies virus(PRV)decreased significantly after the semi-manufactured live virus vaccine was stored at-20℃,four kinds of ice crystal regulators with different concentrations were added to PRV suspension,and the titer loss before and after freezing and thawing was detected after five freeze-thaw cycles.The virus titer loss of 0.25 mol/L AMG,0.14 mol/L Suc,0.45 mmol/L PVP and 0.40 mol/L Lac groups were 0.20,0.26,0.25,0.31lg TCID_(50)/m L,respectively.0.25 mol/L AMG with the best protective effect was mixed with PRV suspension to prepare the semi-manufactured vaccine,and after 60 days of storage at-20℃,the virus titer loss was less than 0.6 lg TCID_(50)/m L.The results of safety evaluation showed that 0.25 mol/L AMG had no obvious toxicity to mice and cells.The results showed that the semi-manufactured porcine PRV prepared by adding 0.25 mol/L AMG to the virus suspension after freeze-thaw cycle had little loss of titer,and was safe for mice and cells,which provided an important theoretical basis for the optimization of freeze-drying process of virus live vaccine production.

关 键 词：伪狂犬病病毒 活疫苗 冰晶调控剂 冻干工艺优化 

分 类 号：S852.65[农业科学—基础兽医学]