脂联素及其受体在不同年龄牦牛附睾中的表达与分布  

Expression of adiponectin and its receptor in epididymis of yaks at different ages

作　　者：祁正满 崔燕[1,2] 余四九 张虔[1] 马睿 牛悦悦 卢凯 翟国亮 QI Zhengman;CUI Yan;YU Sijiu;ZHANG Qian;MA Rui;NIU Yueyue;LU Kai;ZHAI Guoliang(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Gansu Province Livestock Embryo Engineering Research Center,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]甘肃省牛羊胚胎技术研究中心,甘肃兰州730070

出　　处：《中国兽医科学》2024年第6期824-832,共9页Chinese Veterinary Science

基　　金：国家自然科学基金项目(32372974)。

摘　　要：为研究脂联素(APN)及其受体在不同年龄牦牛附睾中的表达与定位,笔者采集幼年(0.5~1岁)、成年(4~5岁)、老年(7岁)牦牛附睾(头、体、尾),通过实时荧光定量PCR(qRT-PCR)、蛋白免疫印迹(Western-blotting)和免疫组织化学(IHC)方法检测APN及其受体1(APNR1)和受体2(APNR2)在附睾(头、体、尾)中的表达与定位。qRT-PCR结果显示,APN和APNR1在老年牦牛附睾尾中的m RNA转录水平显著高于幼年和成年(P<0.05);APNR2在成年和老年牦牛附睾头、体、尾中的转录水平均呈上升趋势,并且APNR2在老年牦牛附睾尾中的转录水平最高(P<0.05)。Western-blotting结果显示,APN蛋白在幼年牦牛附睾头、体、尾中的表达量呈下降趋势(P<0.05),而在成年和老年牦牛的表达趋势相反(P<0.05),且在老年牦牛附睾尾表达量最高(P<0.05);老年牦牛附睾中APNR1的蛋白表达量显著高于幼年和成年牦牛(P<0.05),且在老年牦牛附睾尾表达量最高;APNR2蛋白在幼年牦牛附睾头中表达量最高,而在成年和老年牦牛附睾头、体、尾中均呈上升趋势,且在老年牦牛附睾尾中表达量最高(P<0.05)。IHC结果显示,APN、APNR1及APNR2分布于牦牛附睾上皮细胞、纤毛、间质和精子中。研究结果表明,APN及其受体在牦牛性成熟前后的表达差异显著,推测其对于牦牛附睾中精子成熟、转运和储存等方面具有重要的意义,本实验为高原环境下对于雄性牦牛生殖调控的研究提供了一定的理论依据。The objective of this study was to examine the expression and localization of adiponectin(APN)and its receptor in the epididymis of yaks in different ages.This study examined the expression and localization of Adiponectin(APN)and its receptor in the epididymis(caput,corpus,and cauda)of young(0.5—1 year old),adult(4—5 year old),and old(7 year old)yaks using real-time fluorescence quantitative PCR(qRT-PCR),Western-blotting(WB),and immunohistochemistry(IHC)methods.The study examined the expression and localization of APN and its receptors,APNR1 and APNR2,in different age groups of yaks using q RT-PCR.The results showed that APN and APNR1 expression levels were significantly higher in the epididymal cauda of aged yaks compared to juvenile and adult yaks(P<0.05).APNR2 levels were significantly higher in the caput and corpus of adult and aged yaks compared to the cauda of aged yaks(P<0.05).Additionally,APNR2 levels were higher in the caput and corpus of adult and aged yaks(P<0.05).In the cauda of aged yak epididymis,the m RNA transcript levels of APN and APNR1 were significantly higher(P<0.05)than those of juvenile and adult.APNR2 showed an increasing trend in the caput,corpus,and cauda of adult and aged epididymis,with the highest transcript level in the cauda of aged epididymis(P<0.05).Western-blotting revealed a decreasing trend of APN protein in the caput,corpus,and cauda of juvenile epididymis(P<0.05),while an opposite trend was observed in the caput,corpus,and cauda of aged yak epididymis(P<0.05).The expression of APNR1 protein was significantly higher in aged epididymis than in juvenile and adult epididymis(P<0.05).Additionally,APNR1 protein expression was highest in aged epididymis(P<0.05).APNR2 protein expression was highest in the caput of juvenile epididymis and showed an increasing trend in the caput,corpus,and cauda of adult and aged epididymis.Finally,APNR2 protein expression was highest in the cauda of aged epididymis(P<0.05).The APNR2 protein exhibited a decreasing trend in the caput,corpus,and caud

关 键 词：牦牛 附睾 脂联素 脂联素受体 

分 类 号：S852.165[农业科学—基础兽医学]