观赏用微型兰花组织培养最佳配方的优化  

Optimization of the Best Formula for Tissue Culture of Ornamental Miniature Orchid

作　　者：张月盈 李枝林[1] 和凤美[1] 李叶芳[1] 王玉英[1] 瞿素萍[2] ZHANG Yueying;LI Zhilin;HE Fengmei;LI Yefang;WANG Yuying;QU Suping(College of Gardening and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201;Flower Research Institute of Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《北方园艺》2024年第13期49-57,共9页Northern Horticulture

基　　金：云南省重大科技专项资助项目(202102AE090052);云南省基础研究资助项目(202001AT070101);云南省创新团队资助项目(202105AE160012)。

摘　　要：以大花蕙兰‘大凤’×豆瓣兰‘太极圣梅’(Cymbidium hybridium‘Dafeng’×C.serratum var.goeringii‘Taijishengmei’)F1代试管苗为试材,采用在培养基中添加不同的有机物质,筛选最适生长的培养基;并在此基础上,在培养基中添加不同色素,研究其对植株生长的影响及观赏效果,以期为兰花微型培养的观赏应用研究提供参考依据。结果表明:在1/2 MS+IBA 0.5 mg·L^(-1)+6-BA 2 mg·L^(-1)+碳粉0.5 g·L^(-1)的培养基中,添加75 mL·L^(-1)椰汁有利于兰花试管苗叶长、叶宽、株高以及鲜质量的增加,对植株生长效果最好;在1/2 MS+IBA 0.5 mg·L^(-1)+6-BA 2 mg·L^(-1)+碳粉0.5 g·L^(-1)+椰汁75 mL·L^(-1)的培养基中添加不同浓度的食用色素红曲红、叶绿素铜钠盐、栀子蓝,经121℃高温灭菌后培养基的颜色均会发生变化。叶绿素铜钠盐浓度为533.3 mg·L^(-1)的彩色培养基植株生长良好,培养基整体观赏效果最佳。The F_1 test-tube seedlings of Cymbidium hybridium 'Dafeng'×C.serratum var.goeringii'Taijishengmei' were used as the test materials.Different organic substances were added to the medium to screen the optimal growth medium.On this basis,different pigments were added to the medium to study their effects on plant growth and ornamental effects,in order to provide reference for the study of ornamental application of orchid microculture.The results showed that in the medium of1/2MS+IBA 0.5 mg ·L^(-1)+6BA 2 mg·L^(-1)+ carbon powder 0.5 g·L^(-1),added 75 mL·L^(-1)coconut juice was benefited to the increased of leaf length,leaf width,plant height and fresh weight of orchid plantlets in vitro,and had the best effect on plant growth.In 1/2MS+IBA 0.5 mg·L^(-1)+6-BA2 mg·L^(-1)+carbon powder 0.5 g·L^(-1)+coconut juice 75 mL·L^(-1) medium added different concentrations of edible pigment monascus red,sodium copper chlorophyllin,gardenia blue,after121℃ high temperature sterilization medium color was changed.The plants grew well in the color medium with the concentration of sodium copper chlorophyllin of 533.3 mg·L^(-1),and the overall ornamental effect of the medium was the best.

关 键 词：兰花 微型培养 培养基 生长 观赏应用 

分 类 号：S682.31[农业科学—观赏园艺]