黄肉猕猴桃‘金实4号’果实采后蒂腐病病原菌的分离及鉴定  

Isolation and Identification of Pathogenic Fungi Causing Post-harvest Stem-end Rot of‘Jinshi No.4'Kiwifruit

作　　者：李忆[1] 尹全[2] 张瑶 孙雷明[4] 庄启国[3] LI Yi;YIN Quan;ZHANG Yao;SUN Leiming;ZHUANG Qiguo(Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066;Institute of Quality Standard and Testing Technology Research,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066;China-New Zealand Belt and Road Joint Laboratory on Kiwifruit/Kiwifruit Breeding and Utilization Key Laboratory of Sichuan Province,Sichuan Province Academy of Natural Resource Science,Chengdu,Sichuan 610015;Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou,Henan 450009)

机构地区：[1]四川省农业科学院植物保护研究所,四川成都610066 [2]四川省农业科学院质量标准与检测技术研究所,四川成都610066 [3]四川省自然资源科学研究院中国-新西兰猕猴桃“一带一路”联合实验室,猕猴桃育种及利用四川省重点实验室,四川成都610015 [4]中国农业科学院郑州果树研究所,河南郑州450009

出　　处：《北方园艺》2024年第13期91-96,共6页Northern Horticulture

基　　金：国家重点研发计划资助项目(2022YFD1600701);四川省科技计划资助项目(2023JDZH0012,2024ZHYS0012,2024YFHZ0227)。

摘　　要：以猕猴桃新品种‘金实4号’采后病果为试材,取果实果蒂病组织分离腐烂病病原菌,按照柯赫氏法则确定分离病株的致病性,同时采用形态学结合分子生物学鉴定技术对病原菌种类进行鉴定,明确黄肉猕猴桃果实采后贮藏期引起腐烂的病菌种类,以期为黄肉猕猴桃采后腐烂病防治研究提供参考依据。结果表明:共有8种软腐病病原菌被鉴定,分别是拟茎点霉菌(Phomopsis sp.)、间座壳菌(Diaporthe cotoneastri)、小孢拟盘多毛孢(Pestalotiopsis microspora)、灰葡萄孢(Botrytis cinerea)、新壳梭孢(Neofusicoccum parvum)、链格孢(Alternaria sp.)、镰刀菌(Fusarium sp.)、葡萄座腔菌(Botryosphaeria dothidea)。Naturally diseased fruit of‘Jinshi No.4'kiwifruit were used as test material.The fungi were isolated from a large number of rotten samples,and were identified through their morphologic characteristics and molecular analysis.Koch's postulate was performed to confirm the identification.The fungi which cause post-harvest stem-end rot of yellow-flesh kiwifruit was identified,in order to provide reference for the research on the prevention and control of post-harvest rot disease of yellowflesh kiwifruit.The results showed that eight different fungi were causable for stem-end rot of kiwifruit.They were Phomopsis sp.,Diaporthe cotoneastri,Pestalotiopsis microspora,Botrytis cinerea,Neofusicoccum parvum,Alternaria sp.,Fusarium sp.and Botryosphaeria dothidea.

关 键 词：‘金实4号’猕猴桃 腐烂病 病原菌 鉴定 

分 类 号：S663.4[农业科学—果树学]