复合微生物除臭液制备及其安全性与除臭效果评估  

Preparation of Compound Microbial Deodorization Solution and Evaluation of Its Safety and Deodorization Effect

作　　者：李春艳[1] 崔志峰 王景春 李春华[1] 贺永明[1] LI Chunyan;CUI Zhifeng;WANG Jingchun;LI Chunhua;HE Yongming(Liaoning Agricultural Technical College,Yingkou 115009,China;Dongying Vocational Institute,Dongying 257091,China)

机构地区：[1]辽宁农业职业技术学院,辽宁营口115009 [2]东营职业学院,山东东营257091

出　　处：《辽宁农业职业技术学院学报》2024年第4期18-23,33,共7页Journal of Liaoning Agricultural Technical College

基　　金：辽宁农业职业技术学院2022年度自然科学课题(Lnz202202)。

摘　　要：为制备复合微生物除臭液并评估其除臭效果和安全性,试验通过特殊营养液、光照厌氧处理从鸡粪样品中富集和扩增培养微生物菌液,从中筛选出最优复合微生物除臭液J9和J10,测定其生长曲线,并进行除臭效果试验和小鼠急性毒性检测。结果表明:J9和J10在扩增培养第2 d进入对数期,在第5~6 d进入稳定期。与不喷洒除臭液的对照组相比,筛选出的最优复合微生物除臭液J9和J10对鸡粪产生的NH_(3)和H_(2)S的去除率可高达82.41%和76.18%,显著(P<0.05)高于不喷洒除臭液的对照组。安全评价结果显示,复合微生物除臭液对小鼠急性经口毒性LD_(50)>40 g/kg体重,属于实际无毒级物质,具有较好的安全性。说明该复合微生物除臭液在实际应用中具有高效的除臭能力和安全性,并易制备。In order to prepare compound microbial deodorization solution and evaluate its deodorization effect and safety,the experiment enriched and amplified microbial bacterial solution from chicken manure samples by special nutrient solution and light anaerobic treatment,and screened out the optimal compound microbial deodorization solution J9 and J10.The growth curve was measured,and the deodorization effect test and acute toxicity test in mice were carried out.The results showed that J9 and J10 entered the logarithmic phase on the 2 nd day of amplification culture,and entered the stable phase on the 5 th to 6 th day.Compared with the control group without deodorization solution,the removal rates of NH_(3),and H_(2)S produced by chicken manure by the optimal compound microbial deodorization solution J9 and J10 were as high as 82.41% and 76.18% respectively,which was significantly(P<0.05)higher than the control group without deodorization solution.The results of safety evaluation showed that the acute oral toxicity LD so of the compound microbial deodorant to mice was>40 g/kg body weight,which belonged to the actual non-toxic substance and had good safety.It showed that the compound microbial deodorization solution had high deodorizing ability and safety in practical application,and it was easy to prepare.

关 键 词：复合微生物除臭液 生长曲线 NH_(3) H_(2)S 急性毒性评价 

分 类 号：S852.6[农业科学—基础兽医学] X713[农业科学—兽医学]