中药合剂对红色毛癣菌的抑菌机制及差异基因分析  

Antibacterial mechanism and differential gene analysis of traditional Chinese medicine mixture on Trichophyton rubrum

作　　者：李丹彤 高璐 尹幸念[3] 吴建美 曲琳 LI Dan-tong;GAO Lu;YIN Xing-nian;WU Jian-mei;QU Lin(Inner Mongolia Medical University,Inner Mongolia 010059,China;Lin'an District Center for Disease Control and Prevention,Hangzhou,Zhejiang 311300,China;The Inner Mongolia Autonomous Region Center for Disease Control and Prevention,Inner Mongolia 010031,China;Inner Mongolia People's Hospital,Inner Mongolia 010017,China)

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010059 [2]临安区疾病预防控制中心,浙江杭州311300 [3]内蒙古疾病预防控制中心,内蒙古呼和浩特010031 [4]内蒙古自治区人民医院,内蒙古呼和浩特010017

出　　处：《毒理学杂志》2024年第3期227-231,226,共6页Journal of Toxicology

基　　金：内蒙古科技计划项目(201802117)。

摘　　要：目的探讨中药合剂(知母、黄连、土槿皮、地肤子、决明子和黄柏)对红色毛癣菌的抑菌机制。方法基于高通量测序技术对该合剂作用于红色毛癣菌后的差异基因进行筛选,并基于GO和KEGG数据库对差异基因进行生物信息学分析。结果该中药合剂通过上调短链脱氢酶、谷胱甘肽转移酶、真菌疏水蛋白等产物的基因以及下调甲醛脱氢酶、乙二醛酶、外排泵等产物的基因来影响红色毛癣菌的解毒、防御、外排以及代谢等过程,主要涉及细胞壁、细胞膜以及物质代谢等的改变。结论中药合剂对红色毛癣菌有着较好的抑制作用,其抑菌机制可能与细胞壁、细胞膜完整性以及物质代谢等途径有关,该研究为开发新的抗真菌药物提供了基础。Objective To explore the antibacterial mechanism of traditional Chinese medicine mixture(Rhizoma anemarrhenae,Rhizoma coptidis,Cortex pseudolaricis,Kochia scoparia,Cassia seed and Phellodendri chinensis cortex)against Trichophyton rubrum.Methods High throughput sequencing technology was used to screen the differential genes after the traditional Chinese medicine mixture acted on Trichophyton rubrum.The differential genes were analyzed by bioinformatics based on GO and KEGG databases.Results The traditional Chinese medicine mixture affects the detoxification,defense,efflux and metabolism of Trichophyton rubrum by up-regulating the genes of short chain dehydrogenase,glutathione transferase,fungal hydrophobic protein and other products,and down-regulating the genes of formaldehyde dehydrogenase,glyoxalase,efflux pump and other products.Its mechanism mainly involves the changes of cell wall,cell membrane and material metabolism.Conclusion The traditional Chinese medicine mixture has a good inhibitory effect on Trichophyton rubrum.The inhibitory effect may be related to cell wall and cell membrane integrity,material metabolism and other pathways.This research provides a basis for the development of new antifungal drugs.

关 键 词：中药合剂 高通量测序技术 红色毛癣菌 抑菌机制 

分 类 号：R379.2[医药卫生—病原生物学] R99[医药卫生—基础医学] R114