细胞外囊泡传递CRISPR/Cas9系统的研究进展  

Research Progress on Extracellular Vesicle Delivery CRISPR/Cas9 System

作　　者：刘琨[1,2] 刘炎青 黄楠 杨希 郎巧利 葛良鹏 方仁东[1] LIU Kun;LIU Yanqing;HUANG Nan;YANG Xi;LANG Qiaoli;GE Liangpeng;FANG Rengdong(International Joint Laboratory of Animal Health and Animal Food Safety,College of Veterinary Medicine,Southwest University,Chongqing 400715,China;Chongqing Academy of Animal Sciences,Chongqing 404100,China)

机构地区：[1]西南大学动物医学院,动物健康与动物性食品安全国际联合实验室,重庆400715 [2]重庆市畜牧科学院,重庆404100

出　　处：《中国生物工程杂志》2024年第6期1-10,共10页China Biotechnology

基　　金：国家生猪技术创新中心项目(NCTIP-XDIC17);国家重点研发计划(2021YFA0805901-2);重庆现代农业产业技术体系(CQMAITS202312)资助项目。

摘　　要：CRISPR/Cas9系统能高效便捷的进行基因编辑,在多个领域深受青睐并广泛使用,其传递方法引起国内外大量研究学者的关注。细胞外囊泡(EVs)作为天然的纳米级载体,来源广泛,是一种极具吸引力的CRISPR/Cas9系统传递载体。与常规病毒载体或非病毒载体相比,EVs在安全性、容量、穿透力、靶向性和改造潜力等方面都具有明显优势,有望成为传递CRISPR/Cas9系统的最佳载体。总结了CRISPR/Cas9系统常见的传递策略和加载方式,将EVs与其他载体进行比较,并对EVs传递CRISPR/Cas9系统的优势及国内外研究进展、应用等进行综述,以期为基因编辑递送领域的发展提供帮助。The CRISPR/Cas9 system,known for its efficient and easy gene-editing capabilities,is widely preferred and extensively used in various fields,attracting considerable attention from researchers both domestically and internationally.Extracellular vesicles,as natural nanoscale carriers with abundant sources,are proving to be a highly attractive delivery vehicle for the CRISPR/Cas9 system.Compared to conventional viral or non-viral vectors,extracellular vesicles offer significant advantages in terms of safety,capacity,penetrability,targeting specificity,and transformative potential.They are poised to become the optimal carriers for delivery of the CRISPR/Cas9 system.This article summarizes common delivery strategies and loading methods for the CRISPR/Cas9 system,contrasts extracellular vesicles with other carriers,and provides a comprehensive review of the advantages,domestic and international research progress,and applications of extracellular vesicles in delivering the CRISPR/Cas9 system.The goal is to contribute to the advancement of the field of gene-editing delivery.

关 键 词：CRISPR/Cas9 基因组编辑 细胞外囊泡 纳米载体 

分 类 号：Q78[生物学—分子生物学]