不同剂量新型复合益生菌制剂治疗严重烧伤大鼠肠屏障损伤效果的对比性探究  

COMPARATIVE STUDY OF THE EFFECT OF DIFFERENT DOSES OF THE NEW COMBINED PROBIOTICS PREPARATION IN THE TREATMENT OF INTESTINAL BARRIER DAMAGE IN RATS WITH SEVERE BURNS

作　　者：殷悦 张勇[2] 康雨欣 张倞 余华庆 钱培培 郝兴霞 刘玲英 YIN Yue;ZHANG Yong;KANG Yuxin;ZHANG Jing;YU Huaqing;QIAN Peipei;HAO Xingxia;LIU Lingying(College of Basc Medicine,Inner Mongolia Medical University,Hohhot 010059,China;Department of Nutrition,The Fourth Medical Center of the PLA General Hospital,Beijing 100142,China;College of Graduate,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]内蒙古医科大学基础医学院,内蒙古呼和浩特010059 [2]解放军总医院第四中心营养科,北京海淀100142 [3]河北北方学院研究生院,河北张家口075000

出　　处：《内蒙古医科大学学报》2024年第2期122-126,共5页Journal of Inner Mongolia Medical University

基　　金：国家自然科学基金青年项目(81701900);中国人民解放军总医院青年自主创新科学基金项目(22QNCZ031)。

摘　　要：目的研究通过对比不同剂量新型复合益生菌制剂对严重烧伤大鼠肠屏障损伤的治疗效果。方法使用94℃水浴造模50%Ⅲ度烧伤大鼠,使用三个剂量(0.5×10^(9)CFU/d、1.5×10^(9)CFU/d和4.5×10^(9)CFU/d)益生菌制剂干预。运用ELISA、HE染色和肠屏障评价等来分别观察不同剂量益生菌对大鼠烧伤的改善作用。结果与假伤组(Sham组)相比,烧伤组(Burn组)大鼠肠绒毛及隐窝结构毁损较为严重,肠绒毛高度显著降低(P<0.05),而益生菌治疗的低、中、高剂量组(P-L、P-M、P-H)则具有不同程度的改善,其中以P-M组最佳。同时P-M组的IL-1β、IL-6、LPS水平则均显著降低以及IL-10水平显著升高,差异有统计学意义(P<0.05)。肠屏障功能评价显示,与Burn组相比,P-L、P-M、P-H组小肠中的二胺氧化酶(DAO)和细菌内毒素水平均显著下降,差异有统计学意义(P<0.05),不同剂量组中P-M组内毒素水平下降效果最为显著,差异有统计学意义(P<0.05)。结论该复合益生菌制剂可显著改善和治疗严重烧伤大鼠肠屏障损伤,其最佳剂量为1.5×10^(9)CFU/d。Objective To investigate the therapeutic effect of different doses of novel combined probiotic preparation for the treatment of intestinal barrier injury in severely burned rats.Methods 50%Ⅲ°burned rats model were established us-ing a 94℃water bath,and burned rats were intervened by three doses(0.5×10^(9) CFU/d,1.5×10^(9) CFU/d and 4.5×10^(9) CFU/d)of probiotic preparations.ELISA,HE staining and intestinal barrier evaluation were used to observe the amelio-rative effects of different doses of probiotics on burns in rats,respectively.Results Compared with the sham group,the intestinal villi and crypt structure of rats in the burn group(Burn)were more severely damaged,and the height of intestinal villi was significantly reduced(P<0.05),whereas the probiotics treatment in the low-,medium-,and high-dose groups(P-L,P-M,and P-H,respectively)showed different degrees of improvement,among which the P-M group was the most effective group.Meanwhile,serum IL-1β,IL-6,and LPS levels were significantly reduced as well as IL-10 levels were significantly increased in the P-M group(P<0.05).Evaluation of the intestinal barrier function showed that the levels of diamine oxidase(DAO)and bacterial endotoxin in the small intestine of the P-L,P-M and P-H groups were significantly reduced com-pared with that of the Burn group(P<0.05),and the endotoxin levels in the P-M group showed the most significant reduction among the different dosages(P<0.05).Conclusions The combined probiotics preparation can significantly improve intes-tinal barrier damage in severely burned rats.The optimal dose was 1.5×10^(9) CFU/d.

关 键 词：烧伤 肠屏障 益生菌 炎症 

分 类 号：R644[医药卫生—外科学]