饲粮添加芦丁对肉仔鸡免疫功能和空肠形态、黏膜屏障、抗氧化功能的影响  

Effects of dietary rutin supplementation on immune function,jejunum morphology,mucosal barrier function and antioxidant capacity of broilers

作　　者：马龙飞 陈顺 庄苏[1] 周斌斌 刘慧娟 张佳琦 王恬[1] 王超[1] MA Longfei;CHEN Shun;ZHUANG Su;ZHOU Binbin;LIU Huijuan;ZHANG Jiaqi;WANG Tian;WANG Chao(College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2024年第4期721-730,共10页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31972598);江苏省苏北科技计划项目(SZ-HA2021041)。

摘　　要：[目的]前期研究发现饲粮添加250~1000 mg·kg^(-1)芦丁可提高21日龄肉鸡生长性能,本试验进一步研究芦丁对其肠道形态结构、黏膜屏障和抗氧化功能的影响。[方法]试验选取1日龄健康且体重(43±1)g相近的雄性AA肉鸡256只,随机分为4组,每组8个重复,每个重复8只鸡。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加250、500和1000 mg·kg^(-1)芦丁。21日龄时每个重复取1只肉鸡屠宰取样测定肠道屏障功能、免疫炎症、抗氧化等相关指标。[结果]与对照组相比,饲粮添加250 mg·kg^(-1)芦丁显著提高了肉仔鸡脾脏器官指数、空肠黏膜总超氧化物歧化酶(T-SOD)活性和总抗氧化能力(T-AOC),以及空肠黏膜中闭合小环蛋白1(ZO-1)、闭锁蛋白(OCLN)、核因子E2相关因子2(Nrf 2)和SOD mRNA的表达水平,同时显著降低了血清肿瘤坏死因子α(TNF-α)和D-乳酸(D-LA)水平、空肠黏膜TNF-αmRNA的表达水平(P T<0.05);与对照组相比,饲粮添加500 mg·kg^(-1)芦丁显著提高了肉仔鸡法氏囊器官指数、空肠VH/CD值,空肠黏膜T-SOD活性和T-AOC,空肠黏膜中B细胞淋巴瘤基因2(Bcl-2)、ZO-1和Nrf 2 mRNA的表达水平,并显著降低了血清TNF-α水平(P T<0.05);与对照组和250、500 mg·kg^(-1)芦丁组相比,饲粮添加1000 mg·kg^(-1)芦丁显著提高了血清中免疫球蛋白M(IgM)和免疫球蛋白G(IgG)含量(P T<0.05);与500 mg·kg^(-1)芦丁组相比,饲粮添加250 mg·kg^(-1)芦丁显著提高了肉仔鸡脾脏器官指数、血清脂多糖(LPS)水平和OCLN mRNA表达水平,并显著降低了血清D-LA水平(P T<0.05);与1000 mg·kg^(-1)芦丁组相比,饲粮添加250 mg·kg^(-1)芦丁显著提高了肉仔鸡脾脏器官指数,同时降低了空肠黏膜TNF-αmRNA的表达水平(P T<0.05);与1000 mg·kg^(-1)芦丁组相比,饲粮添加500 mg·kg^(-1)芦丁显著提高了肉仔鸡空肠黏膜中Bcl-2 mRNA的表达水平,并显著降低了血清中LPS水平(P T<0.05)。[结论]饲粮添加250 mg·kg^(-1)或500[Objectives]Previous studies had found that dietary supplementation of 250-1000 mg·kg^(-1) rutin could improve the growth performance of broilers at 21 days of age.This study further investigated the effects of rutin on jejunal morphology,mucosal barrier function and antioxidant capacity of broilers.[Methods]A total of 2561-day-old male Arbor Acres(AA)broilers with similar body weight(43±1)g were randomly divided into 4 groups with 8 replicates per group and 8 chickens per pen.Broilers in the control group were fed a basal diet,and those in experimental groups were fed the basal diet supplemented with 250,500 and 1000 mg·kg^(-1) rutin,respectively.At 21 days of age,one broiler from each replicate was chosen and slaughtered to analyze the indices of intestinal barrier function,immune inflammation,and antioxidant capacity and other related indices.[Results]Compared with the control group,dietary 250 mg·kg^(-1) rutin significantly increased spleen organ index,total superoxide dismutase(T-SOD)activity and total antioxidant capacity(T-AOC)of jejunal mucosa and closed small cyclic protein-1(ZO-1)in jejunal mucosa of broilers,blocking protein(OCLN),nuclear factor E2-related factor 2(Nrf 2)and SOD mRNA expression levels,while serum tumor necrosis faction-α(TNF-α)and D-lactic acid(D-LA)levels,and the expression of TNF-αmRNA in jejunal mucosa significantly decreased(P T<0.05).Compared with the control group,dietary 500 mg·kg^(-1) rutin significantly increased bursal organ index,jejunum VH/CD value,T-SOD activity and T-AOC in jejunal mucosa,B-cell lymphoma gene-2(Bcl-2),ZO-1 and Nrf 2 mRNA expressions in jejunal mucosa of broilers.The serum TNF-αlevel significantly decreased(P T<0.05).Compared with the control group,250 and 500 mg·kg^(-1) rutin group,dietary 1000 mg·kg^(-1) rutin supplementation significantly increased the serum immunoglobulin M(IgM)and immunoglobulin G(IgG)contents(P T<0.05).Compared with 500 mg·kg^(-1) rutin group,dietary 250 mg·kg^(-1) rutin significantly increased spleen organ index,serum l

关 键 词：肉鸡 芦丁 肠道功能 免疫 抗氧化 

分 类 号：S852.6[农业科学—基础兽医学]