用线粒体COI基因序列分析黄尾鲴养殖群体的遗传结构  

Genetic Structure Analysis of Xenocypris davidi Cultured Population Based on Mitochondrial COI Gene Sequence

作　　者：陈大伟 朱鹏灿 刘士力[2] 干超其 郑建波 蒋文枰[2] 程顺[2] 迟美丽[2] 杭小英[2] 李飞[2] CHEN Dawei;ZHU Pengcan;LIU Shili;GAN Chaoqi;ZHENG Jianbo;JIANG Wenping;CHENG Shun;CHI Meili;HANG Xiaoying;LI Fei(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Healthy Freshwater Aquaculture,Ministry of Agriculture and Rural Affairs/Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province,Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China;Huzhou Rongsheng Fishery Technology Co.Ltd.,Huzhou 313105,China)

机构地区：[1]上海海洋大学水产与生命学院农业农村部淡水水产种质资源重点实验室,上海201306 [2]浙江省淡水水产研究所农业农村部淡水渔业健康养殖重点实验室/浙江省淡水水产遗传育种重点实验室,浙江湖州313001 [3]湖州融晟渔业科技有限公司,浙江湖州313105

出　　处：《水产学杂志》2024年第3期1-6,共6页Chinese Journal of Fisheries

基　　金：华东地区部分水产种质资源鉴定(2022CZZX005);国家淡水水产种质资源库长江流域种质资源分库建设(2022).

摘　　要：为了解人工养殖和选育对黄尾鲴(Xenocypris davidi)遗传多样性的影响,利用线粒体COI基因分析中国湖南的醴陵(LL)、浙江的长兴(CX)、双浦(SP)和吴兴(WX)128尾1~2龄的4个黄尾鲴养殖群体的遗传多样性。结果显示,在539 bp的COI部分序列中,检测到75个变异位点,包括74个简约信息位点,序列中A+T的含量(56.1%)明显高于G+C的含量(43.8%),有明显的(A+T)偏好性。在128尾个体中定义了11种单倍型,单倍型Hap-01个体43尾,为优势单倍型。遗传多样性检测结果显示,4个群体的单倍型多样性指数(Hd)介于0.226~0.605,核苷酸多样性指数(π)介于0.00104~0.02221,其中,WX群体的遗传多样性最为丰富。不同养殖群体间遗传距离为0.0145~0.0986,CX和WX群体的遗传距离最近,LL与SP群体的遗传距离最远。AMOVA分析显示,群体间的遗传分化系数Fst为0.8385(P<0.01),遗传变异主要发生在群体间。NJ系统发生树和遗传距离分析结果表明,4个群体间存在相当高的遗传分化。本研究结果可为黄尾鲴遗传多样性变化的比较研究提供基础数据,并为黄尾鲴增殖放流和资源保护提供一定依据。In order to understand the effects of artificial breeding and selective breeding on genetic diversity of Xenocypris davidi,the genetic structure was investigated in 128 samples of four cultured 1-2 years old populations of X.davidi including Liling(LL)from Hunan,Changxing(CX),Shuangpu(SP)and Wuxing(WX)from Zhejiang in China by using mitochondrial COI gene.The results showed that 75 variable sites were detected in the 539 bp partial sequence of COI,including 74 parsimony informative sites.There was significantly higher content of A+T(56.1%)than that of G+C(43.8%),with obvious(A+T)preference.Eleven haplotypes were defined in 128 individuals,with the dominant Haplotype Hap-01(43 individuals).The results of genetic diversity test showed that the haplotype diversity(Hd)was found to be ranged from 0.226 to 0.605,and the nucleotide diversity(π)from 0.0010 to 0.0222,showing different levels of genetic diversity in the four populations.The genetic distance was ranged from 0.0145 to 0.0986 among different populations,with the closest genetic distance between CX and WX populations and the longest genetic distance between LL and SP populations.AMOVA analysis revealed that the genetic differentiation coefficient Fst between populations was 0.8385(P<0.01),the genetic variation primarily found between populations.NJ phylogenetic tree and genetic distance analysis showed that there was a high genetic differentiation among the four populations.The finding provides basic data for the comparative investigation of genetic diversity changes of X.davidi,and for the proliferation and release of X.davidi and resource protection.

关 键 词：黄尾鲴 遗传多样性 遗传结构 养殖群体 

分 类 号：S917[农业科学—水产科学]