基于UPLC-QqQ-MS/MS测定人参属中药对甲亢大鼠类固醇激素含量的影响  

Quantitative Analysis of Steroid Hormones to Explore the Effects of Ginseng Medicines Base on UPLC-QqQ-MS/MS

作　　者：金墨竹 刘琳琳 窦德强[1] JIN Mo-zhu;LIU Lin-lin;DOU De-qiang(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《质谱学报》2024年第4期540-551,I0004,共13页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金(82073985);辽宁省重点研发计划项目(2023JH2/10300058)。

摘　　要：本研究利用超高效液相色谱-三重四极杆串联质谱(UPLC-QqQ-MS/MS)联用技术定量测定甲亢大鼠血清中肾上腺素和多种类固醇激素,探讨血清中类固醇激素含量与甲状腺功能亢进的相关性,以及不同药性人参属中药红参和人参叶对甲亢高代谢的改善作用。采用主成分分析(PCA)、Spearman检验进行肾上腺素和类固醇激素与甲状腺功能的相关性分析。结果表明,UPLC-QqQ-MS/MS能够快速检测待测物的含量和变化,灵敏度高、重现性好、准确率高且分析速度快。与空白组相比,甲亢大鼠血清中类固醇激素含量均有不同程度的降低,给予不同药性人参属中药后均出现升高。类固醇激素水平异常在一定程度上与甲状腺激素有关,皮质酮和肾上腺素可作为中药调节类固醇激素代谢的关键指标;睾酮可作为寒性中药调节类固醇激素的生物标志物;肾上腺素和雄烯二酮可作为温性中药调节类固醇激素的生物标志物。该方法能够系统、快速、准确、高效地完成大鼠血清中类固醇激素含量的测定,为类固醇激素的分析测定提供方法参考。The medicinal nature of traditional Chinese medicine(TCM)mainly plays a role in regulating the energy and material metabolism of the body.The energy metabolism of the body is also regulated by the nervous and endocrine systems,and the hormones are an important component of the endocrine system to realize the coordinated action.Therefore,the medicinal nature of TCM is inextricably linked with the metabolism of hormones.Hyperthyroidism,qi-yin deficiency syndrome is regarded as the classic heat syndrome category in Chinese medicine,which can be used as model to reflect the mechanism and difference of qi tonifying of ginseng herbs with different medicinal natures.Steroid hormones are synthesized in the adrenal cortex,mitochondria of placental cells,smooth endoplasmic reticulum,and gonads of the human body by the participation of various metabolic enzymes.Meanwhile,most of the patients with hyperthyroidism have adrenocortical dysfunction in the clinic,and the corresponding clinical symptoms are closely related to the hypothalamic-pituitary-thyroid axis(HPT),the hypothalamic-pituitary-adrenal axis(HPA),and the hypothalamic-pituitary-gonadal axis(HPG).When hyperthyroidism occurs,the production and release of steroid hormones will be affected to a certain extent.To explore the correlation between the level of steroid hormones and hyperthyroidism,and the ameliorative effects of red ginseng and ginseng leaves of different types of the genus Ginseng on hypermetabolism of hyperthyroidism and the changes of related indexes,a method of ultra-high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry(UPLC-QqQ-MS/MS)was established for rapid identification and quantification of chemical components,including adrenaline,androstenedione,testosterone,dehydroepiandrosterone,estriol,progesterone,17α-hydroxypregnenolone,corticosterone,and cortisol in rat serum.Then,multivariate analysis method,principal component analysis(PCA),and Spearman test were used to analyze the correlation among adrenaline,ste

关 键 词：甲亢 肾上腺素 类固醇激素 超高效液相色谱-三重四极杆串联质谱(UPLC-QqQ-MS/MS) 红参 人参叶 定量测定 

分 类 号：O657.63[理学—分析化学]