益生菌结合蒙脱石散对轮状病毒感染乳鼠肠道黏膜、肠道微生物功能相关基因表达的影响  

Effects of probiotics combined with montmorillonite powder on intestinal mucosa and expressions of intestinal microorganism function-related genes in neonatal rats with rotavirus infection

作　　者：赵林 徐沙沙 郭伟胜[1] 刘鹏[1] 席作武[2] ZHAO Lin;XU Sha-sha;GUO Wei-sheng;LIU Peng;Ta XI Zuo-wu(Second Department of General Surgery,Henan Provincial Hospital of Traditional Chinese Medicine/The Second Affiliated Hospital of Henan Universiytyof Traditional Chinese Medicine,Zhengzhou 450002,Henan Province,China;Department of Anorectal,Henan Provincial Hospital of Traditional Chinese Medicine/The Second Affiliated Hospital of Henan Universiytyof Traditional Chinese Medicine,Zhengzhou 450002,Henan Province,China;Department of Respiratory,1Henan Provincial Children's Hospital,Zhengzhou 450018,Henan Province,China)

机构地区：[1]河南省中医院、河南中医药大学第二附属医院普外二科,河南郑州450002 [2]河南省中医院、河南中医药大学第二附属医院肛肠科,河南郑州450002 [3]河南省儿童医院呼吸内科,河南郑州450018

出　　处：《中国临床药理学杂志》2024年第13期1903-1907,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家中医药管理局中医药科学技术研究专项课题基金资助项目(GZY-KJS-2022-041-1)。

摘　　要：目的分析益生菌结合蒙脱石散对轮状病毒(RV)感染乳鼠肠道黏膜、肠道微生物功能相关基因表达的影响。方法用灌胃SA11株轮状病毒构建RV感染模型。将48只乳鼠随机分为正常组(0.9%NaCl)、模型组(0.9%NaCl)、实验组(0.06 g·mL^(-1)蒙脱石散)和联合组(0.06 g·mL^(-1)蒙脱石散+6.5×10~7 CFU·mL^(-1)布拉氏酵母菌散),每组12只。4组乳鼠每天给药1次,连续给药5 d。收集粪便进行评价,用酶联免疫吸附试验法检测血清肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)和IL-17水平,用实时荧光定量聚合酶链反应法检测肠道组织中水通道蛋白(AQP)水平,用细菌16srDNA荧光定量聚合酶链反应法检测粪便中双歧杆菌和大肠埃希菌含量。结果正常组、模型组、实验组和联合组的粪便评分分别为(1.01±0.10)、(2.97±0.08)、(2.84±0.03)和(2.77±0.03)分,TNF-α水平分别为(132.54±14.63)、(185.66±19.64)、(165.25±17.63)和(149.95±15.76)pg·mL^(-1),IL-1β水平分别为(172.32±18.68)、(265.34±27.72)、(202.34±21.34)和(186.24±19.46)pg·mL^(-1),IL-17水平分别为(118.62±12.44)、(173.24±18.25)、(152.32±16.72)和(122.54±13.58)pg·mL^(-1),AQP2 mRNA表达水平分别为1.02±0.05、0.72±0.07、0.89±0.08和1.21±0.12,AQP4 mRNA表达水平分别为1.04±0.07、0.42±0.05、0.78±0.08和1.19±0.12,AQP8 mRNA表达水平分别为1.00±0.06、0.63±0.06、0.91±0.09和1.30±0.13,双歧杆菌含量分别为(6.35±0.64)、(4.31±0.44)、(4.93±0.50)和(5.34±0.54)CFU·g^(-1),大肠埃希菌含量分别为(6.14±0.62)、(8.78±0.88)、(8.46±0.85)和(8.12±0.83)CFU·g^(-1)。模型组的上述指标和实验组、联合组、正常组比较,在统计学上差异均有统计学意义(均P<0.05)。结论蒙脱石散联合益生菌干预RV感染乳鼠可改善粪便性状,降低血清炎症因子水平,纠正肠道菌群紊乱,其可能是通过改善肠道AQP2、AQP4和AQP8表达实现的。Objective To analyze the effects of probiotics combined with montmorillonite powder on intestinal mucosa and expressions of intestinal microorganism function-related genes in neonatal rats with rotavirus(RV)infection.Methods RV infection model was established by intragastric administration of SA11 strain rotavirus.Forty-eight suckling rats were randomly divided into normal group(0.9%NaCl),model group(0.9%NaCl),experimental group(0.06 g·mL^(-1)montmorillonite powder)and combined group(0.06 g·mL^(-1)montmorillonite powder+6.5×10~7 CFU·mL^(-1)Saccharomyces cerevisiae powder)with 12 rats in each group.The feces were collected for evaluation.The levels of serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-17 were detected by enzyme-linked immunosorbent assay,the levels of aquaporin(AQP)in intestinal tissues was detected by real-time fluorescence quantitative polymerase chain reaction,and the counts of Bifidobacteria and Escherichia coli in feces were detected by bacterial 16srDNA fluorescence quantitative polymerase chain reaction.Results The feces scores in normal,model,experimental and combined groups were(1.01±0.10),(2.97±0.08),(2.84±0.03)and(2.77±0.03)points;TNF-αlevels were(132.54±14.63),(185.66±19.64),(165.25±17.63)and(149.95±15.76)pg·mL^(-1);IL-1βlevels were(172.32±18.68),(265.34±27.72),(202.34±21.34)and(186.24±19.46)pg·mL^(-1);IL-17 levels were(118.62±12.44),(173.24±18.25),(152.32±16.72)and(122.54±13.58)pg·mL^(-1);Bifidobacteria counts were(6.35±0.64),(4.31±0.44),(4.93±0.50)and(5.34±0.54)CFU·g^(-1);Escherichia coli counts were(6.14±0.62),(8.78±0.88),(8.46±0.85)and(8.12±0.83)CFU·g^(-1);mRNA levels of AQP2 were 1.02±0.05,0.72±0.07,0.89±0.08 and 1.21±0.12;mRNA expression levels of AQP4 were1.04±0.07,0.42±0.05,0.78±0.08 and 1.19±0.12;mRNA expression levels of AQP8 were 1.00±0.06,0.63±0.06,0.91±0.09 and 1.30±0.13,respectively.There were significant differences of above indexes between the model group with the normal,experimental and combined groups(al

关 键 词：益生菌 蒙脱石散 轮状病毒 肠道黏膜 肠道微生物 

分 类 号：R97[医药卫生—药品]