补肾活血方对复发性流产大鼠IL-17的影响  

Effects of Bushen Huoxue formula on interleukin-17 in rats with recurrent spontaneous abortion

作　　者：彭瑶[1] 陈瑾[1] 梁艳[1] 杨琦 沈丽梅 夏晓健 PENG Yao;CHEN Jin;LIANG Yan;YANG Qi;SHEN Li-mei;XIA Xiao-jian(Department of Traditional Chinese Medicine Gynecology,Jiangxi Maternal andChildHealth Hospital,Jiangxi Integrated Traditional lChineseand Western Medicine Gynecological Disease Diagnosis and Treatment Center,Jiangxi Provincial Key Researchh Laboratory of Integrated Traditional Chinese and Western Medicinen Female Reproduction,Nanchang 330006,Jiangxi Province,China;Graduate School,Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]江西省妇幼保健院、江西省中西医结合妇科病诊疗中心、江西省中西医结合女性生殖重点研究室中医妇科,江西南昌330006 [2]江西中医药大学研究生院,江西南昌330006

出　　处：《中国临床药理学杂志》2024年第13期1928-1932,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家中医药管理局第七批全国名老中医药专家学术经验继承工作资助项目(国中医药人教函[2022]76号);廖彩森全国名老中医药专家传承工作室(国中医药人教函[2022]75号);江西省卫生健康委科技计划基金资助项目(SKJP220218590);中国妇幼保健协会-华润三九中医中西医结合妇幼健康专项研究基金资助项目(ZXY2020B06)。

摘　　要：目的观察补肾活血方对复发性流产(RSA)大鼠白细胞介素-17(IL-17)的治疗作用。方法用腹腔注射雌二醇苯甲酸酯的方法构建RSA模型大鼠。将40只RSA模型随机分为模型组、对照组和低、高剂量实验组,每组10只;另取10只健康孕鼠设为空白组。低、高剂量实验组分别灌胃含7.77、15.54 g·kg^(-1)生药的补肾活血方溶液;对照组灌胃含2.10 mg·kg^(-1)地屈孕酮的溶液;空白组和模型组均灌胃等量0.9%NaCl。5组大鼠的给药剂量均为10 mL·kg^(-1),每天1次,连续给药9 d。观察各组活胎数、胚胎丢失数、胚胎丢失率,用流式细胞仪检测外周血中Th17细胞构成比,用蛋白质印迹法检测蜕膜组织中IL-6、IL-17、IL-23蛋白的表达水平。结果高剂量实验组、对照组、模型组和空白组的活胎数分别为(11.50±2.84)、(11.50±3.10)、(6.30±1.25)和(12.50±3.24)个,胚胎丢失数分别为(1.80±0.42)、(1.90±0.57)、(4.90±1.37)和0个,胚胎丢失率分别为(14.01±4.52)%、(14.79±6.06)%、(43.50±9.49)%和0个,外周血Th17细胞构成比分别为(3.12±0.47)%、(3.10±0.59)%、(5.31±1.16)%和(2.54±0.71)%,IL-6蛋白相对表达水平分别为0.19±0.04、0.18±0.05、0.85±0.16和0.11±0.03,IL-17蛋白相对表达水平分别为0.28±0.04、0.29±0.05、0.84±0.12和0.09±0.01,IL-23蛋白相对表达水平分别为0.35±0.04、0.34±0.06、0.90±0.11和0.08±0.01。模型组的上述指标与高剂量实验组、对照组和空白组比较,在统计学上差异均有统计学意义(均P<0.05)。结论补肾活血方可减少RSA大鼠胚胎丢失、改善蜕膜组织病理变化,降低Th17细胞构成比和其相关细胞因子IL-6、IL-17、IL-23的表达。Objective To observe the effect of Bushen Huoxue formula on interleukin-17(IL-17)in rats with recurrent spontaneous abortion(RSA).Methods RSA model rats were constructed by intraperitoneal injection of estradiol benzoate.Forty RSA model rats were randomly divided into model group,control group and experimental-L,-H groups,with 10 rats per group.Another 10 healthy pregnant rats were set as blank group.The experimental-L,-H groups were given 7.77 and15.54 g·kg^(-1)of Bushen Huoxue formula solution by gavage.The control group was given 2.10 mg·kg^(-1)of dydrogesterone solution by gavage.The blank group and the model group were given equal amount of0.9%NaCl by gavage.The dose of administration for the five groups was10 mL·kg^(-1),once a day,for 9 days.The number of live fetuses,embryo loss,and embryo loss rate in each group were observed.The proportion of Th17 cells in the peripheral blood was detected by flow cytometry.The expression levels of interleukin-6(IL-6),IL-17 and IL-23 proteins in the decidual tissues were detected by Western blotting.Results The number of live fetuses in the experimental-H,control,model and blank groups were 11.50±2.84,11.50±3.10,6.30±1.25 and 12.50±3.24;the number of embryos lost were 1.80±0.42,1.90±0.57,4.90±1.37 and 0;the rates of embryo loss were(14.01±4.52)%,(14.79±6.06)%,(43.50±9.49)%and 0;the proportions of Th 17 cells in the peripheral blood were(3.12±0.47)%,(3.10±0.59)%,(5.31±1.16)%and(2.54±0.71)%;the relative expression levels of IL-6protein were 0.19±0.04,0.18±0.05,0.85±0.16 and 0.11±0.03;the relative expression levels of IL-17protein were 0.28±0.04,0.29±0.05,0.84±0.12 and 0.09±0.01;the relative expression levels of IL-23protein were 0.35±0.04,0.34±0.06,0.90±0.11 and 0.08±0.01,respectively.Comparing experimental-H group and model group,comparing control group and blank group,the above indexes were statistically significant(all P<0.05).Conclusion Bushen Huoxue formula can reduce embryo loss,improve placental tissue pathology,reduce Th17 cell proporti

关 键 词：补肾活血方 复发性流产 白细胞介素-17 胚胎丢失 

分 类 号：R28[医药卫生—中药学]