丝素蛋白搭载中药复方覆膜肠道支架的制备与性能研究  

Preparation and properties of coated intestinal stents loaded with traditional Chinese medicine compound by silk fibroin

作　　者：陈刘彬 冯裕胜 孙玮玮 游玮羿 汪文静 陈鸿 李刚 韩植芬[4] Chen Liubin;Feng Yusheng;Sun Weiwei;You Weiyi;Wang Wenjing;Chen Hong;Li Gang;Han Zhifen(College of Textile and Clothing Engineering,Soochow University,Suzhou 215123,Jiangsu,China;National Engineering Laboratory for Modern Silk,Soochow University,Suzhou 215123,Jiangsu,China;Department of General Surgery,The Fourth Affiliated Hospital of Soochow University,Suzhou 215000,Jiangsu,China;Department of Integrated Traditional Chinese and Western Medicine,The Fourth Affiliated Hospital of Soochow University,Suzhou 215000,Jiangsu,China)

机构地区：[1]苏州大学纺织与服装工程学院,江苏苏州215123 [2]苏州大学现代丝绸国家工程实验室,江苏苏州215123 [3]苏州大学附属第四医院普外科,江苏苏州215000 [4]苏州大学附属第四医院中西医结合科,江苏苏州215000

出　　处：《产业用纺织品》2024年第6期1-10,共10页Technical Textiles

基　　金：国家自然科学基金面上项目(82374295);国家重点研发计划(2021YFE0111100);科技部对发展中国家常规性科技援助项目(KY202201002);江苏省科技计划专项(BZ2022017)。

摘　　要：采用丝素蛋白搭载中药复方提取物的方法,通过纬编技术与静电纺丝工艺的结合,制备具有可降解性能的载药覆膜肠道支架。选取聚对二氧环己酮(polydioxanone, PDO)单丝,采用小口径圆纬织机制备覆膜肠道支架的内层。采用静电纺丝工艺对内层肠道支架进行覆膜,制备不同参数的核-壳结构载药纳米纤维膜。其中,纺丝乳液的油相为聚己内酯(polycaprolactone, PCL)溶液,水相由丝素蛋白(silk fibroin, SF)和中药复方提取物于去离子水中共混而成,所搭载的中药复方由补骨脂、黄芪、野葡萄藤、山慈菇、炒白术和地黄组成。分别测试了纺丝乳液的稳定性、黏度、电导率和制备的纳米纤维膜的表面形貌。结果显示:纤维直径与中药复方质量分数成正比;随着PCL质量分数和中药复方质量分数的增加,纳米纤维直径增加;纤维连续光滑,无串珠、折叠、破损等情况出现,载药纳米纤维膜的性能良好。A biodegradable drug-loaded coated intestinal stent was prepared by the combination of weft knitting technology and electrospinning technology using silk fibroin protein loaded with extracts of traditional Chinese medicine(TCM)compound.Polydioxanone(PDO)monofilament was selected and the inner woven layer of the coated intestinal stent was prepared by using a small diameter cylindrical weft knitting machine.The inner intestinal stent was coated by electrospinning technology to prepare core-shell drug-loaded nanofiber drug films with different parameters.The oil phase of the spinning emulsion was made of polycaprolactone(PCL)solution,and the water phase was silk fibroin(SF)and TCM compound extract mixed in deionized water.The TCM compound was composed of psoralea,Astragalus,wild grape vine,mountain mushroom,fried white art and rehmannia.The stability,viscosity and electrical conductivity of the spinning emulsion and the surface morphology of the prepared nanofiber films were tested.The results showed that the fiber diameter was directly proportional to the mass fraction of the TCM compound.With the increase of PCL mass concentration and mass fraction of TCM compound,the nanofiber diameter increased.The fibers were continuous and smooth,without beading,folding,breaking,etc.,and the mechanical properties performance of nanofiber loaded drug drug-loaded nanofiber film was good.

关 键 词：肠道支架 纬编 静电纺丝 丝素蛋白 中药复方 

分 类 号：TS141.8[轻工技术与工程—纺织材料与纺织品设计]