Box-Behnken法优化锦灯笼的提取工艺及其体外活性评价  

Optimization of Extraction Process Physalis alkekengi L.var.franchetii(Mast.)Makino by Box-Behnken Response Surface Method and in vitro Evaluation

作　　者：侯梦婷 朱玥 李飘羽 于洋 HOU Meng-ting;ZHU Yue;LI Piao-yu;YU Yang(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Liaoning Dalian 116620,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116620

出　　处：《广州化工》2024年第9期74-77,100,共5页GuangZhou Chemical Industry

基　　金：辽宁中医药大学高层次引进人才科研启动资金资助项目(2100221005);2023年度辽宁省自然科学基金博士启动(2023-BS-139);辽宁中医药大学2022年度大学生创新创业训练计划立项项目(X202210162024)。

摘　　要：以总黄酮和总皂苷的综合评分为考察指标,采用Box-Behnken法优化提取工艺最佳的乙醇浓度、提取时间、料液比及浸泡时间。考察提取物的ABTS+及DPPH清除率及对在H 2 O 2诱导的氧化损伤B16细胞存活率,SOD、CAT和MDA水平评价其抗氧化活性。结果显示,最佳提取工艺为:13.45倍量67.86%乙醇溶液浸泡0.89 h,回流1.68 h提取3次。锦灯笼提取物具有良好的抗氧化活性,可提高细胞CAT和SOD活性,降低MDA含量;提高氧化损伤状态的B16细胞的存活率,为深入研究锦灯笼提供实验基础。Using the comprehensive scores of total flavonoids and total saponins as the evaluation indicators,the Box Behnken method was used to optimize the extraction process for the optimal ethanol concentration,extraction time,solid-liquid ratio and soaking time.The ABTS+and DPPH clearance rates of the extract were assessed,as well as its antioxidant activity against H 2 O 2 induced oxidative damage to B16 cells and the levels of SOD,CAT,and MDA.The results showed that the optimal extraction process was as follows:13.45 times the amount of 67.86%ethanol solution soaked for 0.89 h,refluxed for 1.68 h,and extracted three times.Jindeng Lantern extract had good antioxidant activity,which can increase cellular CAT and SOD activities and reduce MDA content.Improving the survival rate of B16 cells in oxidative damage state provided an experimental basis for in-depth research on Jindeng lanterns.

关 键 词：锦灯笼 Box-Behnken法 提取 黄酮 皂苷 抗氧化 B16细胞 

分 类 号：R284.2[医药卫生—中药学]