不同给药方式的虎杖苷对实验性矽肺大鼠模型的干预效果  

Comparison of interventional effects of polydatin on experimental silicosis rats by different administration methods

作　　者：吴冰冰 汤旖雯 张笑璇 赵丽媛 沈曦 沈福海 WU Bingbing;TANG Yiwen;ZHANG Xiaoxuan;ZHAO Liyuan;SHEN Xi;SHEN Fuhai(Hebei Coal Mine Health and Safety Laboratory,School of Public Health,North China University of Science and Technology,Tangshan,Hebei 063210,China)

机构地区：[1]华北理工大学公共卫生学院/河北省煤矿卫生与安全实验室,河北唐山063210

出　　处：《职业卫生与应急救援》2024年第3期397-402,共6页Occupational Health and Emergency Rescue

基　　金：Q27植物芯对小鼠尘肺模型的预防与治疗作用及其毒理学研究(企业委托项目)。

摘　　要：目的比较虎杖苷在不同给药方式下对实验性矽肺大鼠模型的干预效果。方法无特定病原体(specific pathogen free,SPF)级雄性SD大鼠50只,按随机数字表法分为对照组、二氧化硅模型组、虎杖苷雾化吸入组、虎杖苷灌胃组和虎杖苷腹腔注射组5组,每组10只。其中对照组不予任何处理,模型组在给予二氧化硅造模后不再给予处理,其他3组在二氧化硅造模后立即分别以不同的给药方式使用虎杖苷进行干预。观察给药后第28天、第56天各组大鼠一般情况以及肺系数。取大鼠肺组织制备石蜡切片并进行苏木精-伊红(hematoxylin-eosin staining,HE)及马松(Masson)染色,并应用试剂盒检测各组大鼠肺组织中羟脯氨酸和血清中丙二醛浓度,评价各组大鼠肺部炎症及肺纤维化程度。结果在给药后第56天,与对照组相比,模型组大鼠肺系数明显升高(P<0.01);与模型组相比,腹腔注射组大鼠肺系数有所降低(P<0.05)。各组大鼠肺组织HE染色结果表明,第28天和第56天对照组肺泡壁薄,肺泡结构正常,均未见塌陷;与第28天对照组相比,第28天模型组肺泡壁明显增厚、肺泡间隔增宽,肺泡腔有大量炎症细胞浸润;与第28天模型组相比,虎杖苷各干预组肺泡结构相对正常。与第56天对照组相比,第56天模型组肺泡壁进一步增厚;与第56天模型组相比,腹腔注射组、灌胃组、雾化吸入组肺泡间距减小。大鼠肺组织Masson染色结果表明,与对照组相比,第28天、第56天模型组肺组织均有蓝色条索状胶原纤维增生。胶原容积分析结果表明,在第28天、第56天,与对照组相比,模型组的胶原容积分数明显上升(P<0.05);与模型组相比,腹腔注射组、灌胃组、雾化吸入组胶原容积分数有所下降(P<0.01),其中腹腔注射组胶原容积分数下调水平优于其余两组。在给药后第56天,与对照组相比,其他各组肺组织中羟脯氨酸水平均有所升高(P<0.05);与模型组相Objective The aim of this study was to compare the interventional effects of polydatin given by different administration modes on experimental silicosis rats.Methods Fifty SPF SD rats were randomly divided into five groups,namely the control group,silica model group,polydatin inhalation group,polydatin gavage group,and polydatin intraperitoneal injection group.There were 10 rats in each group.Among them,the control group did not have any treatment,the model group was only treated with silica molding,and the other 3 groups were treated with polydatin via different administration methods after silica molding.The general condition of rats in each group and the lung coefficient of each group were observed at 28 days and 56 days after dust treatment.Paraffin sections of rat lung tissue were stained with hematoxylin-eosin(HE)and Masson staining.The kit was used to detect the content of hydroxyproline(HYP)and malondialdehyde(MDA)in the lung tissue of rats in each group,and the degree of lung inflammation and pulmonary fibrosis in each group of rats was evaluated.Results At day 56 after dust exposure,the lung coefficient of rats in the model group was significantly increased(P<0.001)compared with the control group;compared with the model group,the lung coefficient of rats in the intraperitoneal injection group was decreased(P<0.05).The results of HE staining of lung tissue of rats in each group showed that the alveolar wall was thin and the alveolar structure was normal in the control group on the 28th and 56th days,and no collapse was seen;compared with the control group on the 28th day,the alveolar wall of the rats in the 28th day model group was significantly thickened,the alveolar septum was widened,and a large number of inflammatory cells infiltrated the alveolar cavity;compared with the 28th day model group,the alveolar structure of each intervention group of polydatin was relatively normal.At day 56,compared with the control group,the alveolar wall of fibroblasts and fibroblast hyperplasia were further thickened i

关 键 词：虎杖苷 矽肺 腹腔注射 灌胃 雾化吸入 羟脯氨酸 丙二醛 

分 类 号：R135.2[医药卫生—劳动卫生] R114[医药卫生—公共卫生与预防医学]