肠道分节丝状菌与儿童轮状病毒感染关系分析  

Analysis of the relationship between intestinal segmented filamentous bacteria and rotavirus infection in children

作　　者：杨婷 陈波[1] 龙高 舒小莉[1] 江米足[2] Yang Ting;Chen Bo;Long Gao;Shu Xiaoli;Jiang Mizu(Gastrointestinal Laboratory,Children′s Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,National Children′s Regional Medical Center,Hangzhou 310052,China;Department of Gastroenterology and Pediatric Endoscopy Center,Children′s Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,National Children′s Regional Medical Center,Hangzhou 310052,China;Department of Pediatrics,Taizhou Central Hospital(Taizhou University Hospital),Taizhou 318015,China)

机构地区：[1]浙江大学医学院附属儿童医院消化实验室、国家儿童健康与疾病临床医学研究中心、国家儿童区域医疗中心,杭州310052 [2]浙江大学医学院附属儿童医院消化科和儿童内镜中心、国家儿童健康与疾病临床医学研究中心、国家儿童区域医疗中心,杭州310052 [3]浙江省台州市中心医院(台州学院附属医院)儿科,台州318015

出　　处：《中华儿科杂志》2024年第7期643-648,共6页Chinese Journal of Pediatrics

基　　金：国家儿童健康与疾病临床医学研究中心项目(G20A0008);浙江省医药卫生重大科技计划(WKJ-ZJ-1622)。

摘　　要：目的探讨肠道分节丝状菌(SFB)定植与轮状病毒感染风险的关系及SFB抵御轮状病毒感染的可能机制。方法病例对照研究。选择在浙江大学医学院附属儿童医院门诊因腹泻经粪便检测轮状病毒阳性的0~5岁患儿50例为研究对象,即轮状病毒性肠炎组,并以非胃肠道疾病、非感染性疾病的外科疾病患儿55例为对照组,年龄、性别组成与轮状病毒性肠炎组相匹配。提取粪便菌群的DNA,通过实时荧光定量PCR分析测定SFB,将轮状病毒性肠炎组及对照组患儿分别按照年龄和性别分组,分析SFB的阳性率在不同组间的差异,并进一步比较分析这两组患儿在≤2岁组及>2~5岁年龄组SFB阳性率的差异。通过p3340蛋白与轮状病毒进行中和试验,确定Vero细胞轮状病毒感染率与p3340浓度之间的关系。组间比较采用χ^(2)检验或Fisher确切概率法。结果轮状病毒性肠炎组患儿50例,年龄(1.7±0.9)岁,对照组患儿55例,年龄(1.8±1.1)岁。轮状病毒性肠炎组患儿SFB的阳性率在各个年龄中呈递减分布,其中≤1岁组最高,为10/14,>1~2岁组为67%(14/21),>2~5岁组为9/15,差异无统计学意义(P=0.867);对照组患儿≤1岁组SFB阳性率为12/15,>1~2岁组为95%(19/20),>2~5岁组为50%(10/20),差异有统计学意义(P=0.004)。轮状病毒性肠炎组患儿男童SFB阳性率为74%(20/27),女童为56%(13/23),差异无统计学意义(χ^(2)=1.71,P=0.192);对照组患儿男童SFB阳性率为79%(22/28),女童为70%(19/27),差异无统计学意义(χ^(2)=0.49,P=0.485)。轮状病毒性肠炎组SFB阳性率66%(33/50),对照组为75%(41/55),差异无统计学意义(χ^(2)=0.56,P=0.454)。在≤2岁患儿中,轮状病毒性肠炎组SFB阳性率69%(24/35),对照组为89%(31/35),差异有统计学意义(χ^(2)=4.16,P=0.041);在>2~5岁患儿中,轮状病毒性肠炎组SFB阳性率为9/15,对照组为50%(10/20),差异无统计学意义(P=0.734)。经Pearson相关性分析,随着SFB阳性率的增加,轮状病毒的感染率呈�Objective To investigate the association between intestinal colonization of segmented filamentous bacteria(SFB)and the risk of rotavirus infection,and the possible mechanisms by which SFB resist rotavirus infection.Methods This case-control study enrolled 50 children aged 0 to 5 years who present to the outpatient Department of Children′s Hospital,Zhejiang University School of Medicine with diarrhea and positive stool tests for rotavirus.The children were divided into rotavirus enteritis group and control group consisting of 55 children with non-gastrointestinal and non-infectious surgical diseases.The age and sex composition of the two groups was matched.The DNA of the fecal flora was extracted and SFB was detected by real-time fluorescence quantitative PCR analysis.The children in the rotavirus enteritis group and the control group were subgrouped by age and sex to analyze the differences in SFB positivity rates between different groups,and further compare and analyze the differences in SFB positivity rates between these two groups of children in the≤2 years old subgroup and the>2-5 years old subgroup.Neutralization test was performed with p3340 protein and rotavirus to determine the relationship between rotavirus infection rate and p3340 concentration in Vero cells.χ^(2) test or Fisher′s exact probability method was used for comparison between the two groups.Results There were 50 children in the rotavirus enteritis group with an age of(1.7±0.9)years,and 55 children in the control group with an age of(1.8±1.1)years.The positive rate of SFB in children with rotavirus enteritis showed a declining trend across ages groups,with the highest rate of 10/14 in the≤1 year old group,followed by 67%(14/21)in the>1-2 years old group,9/15 in the>2-5 years old group,and there was no statistically significant difference(P=0.867).The positive rate of SFB in the control group was 12/15 in the≤1 year old group,95%(19/20)in the>1-2 years old group,50%(10/20)in the>2-5 years old group,with statistical significance(P=0

关 键 词：轮状病毒属 腹泻 粪便 儿童 

分 类 号：R725.1[医药卫生—儿科]