CO_(2)刺激响应球型水凝胶的制备及在蛋白质分离中的应用  

Preparation of CO_(2) Stimulus-Responsive Hydrogel and Its Application in Protein Separation

作　　者：张守村[1] 闫雪垠 宋阳华 李卓敏 ZHANG Shou-Cun;YAN Xue-Yin;SONG Yang-Hua;LI Zhuo-Min(Key Laboratory of Polymer Materials and Manufacturing Technology,College of Materials Science and Engineering,North Minzu University,Yinchuan 750021)

机构地区：[1]北方民族大学材料科学与工程学院,高分子材料及制造技术重点实验室,银川750021

出　　处：《应用化学》2024年第6期870-877,共8页Chinese Journal of Applied Chemistry

基　　金：省部共建“粉体材料及特种陶瓷”重点实验室项目(No.2201);宁夏自然科学基金项目(No.2023AAC03282)资助。

摘　　要：以纤维素、甲基丙烯酸二甲基胺基乙酯和N,N'-亚甲基双丙烯酰胺等为原料制备了纤维素/聚甲基丙烯酸二甲基胺基乙酯互穿网络复合水凝胶材料(GCel/PD)。GCel/PD材料具有显著的CO_(2)刺激响应特性,能有效地吸附分离牛血清白蛋白(BSA)。其中,对BSA的最大吸附量可达(434.4±15)mg/g。在CO_(2)/N2氛围的循环切换下,GCel/PD材料表现出良好的循环稳定性,在循环使用3次之后,对蛋白质的吸附量仍能达到167.5 mg/g。使用之后,颗粒状GCel/PD便于从体系中分离出来,有望在蛋白质的分离提取方面得到应用。A composite hydrogel material,denoted as GCel/PD,is synthesized by utilizing cellulose,N,N'-dimethylaminoethyl methacrylate(DMAEMA)and N,N'-methylenebis(acrylamide)as the main raw materials.The results demonstrate that the GCel/PD materials exhibit significant CO_(2) stimulus-responsive characteristics,and can effectively adsorb and separate bovine serum albumin(BSA).The maximum adsorption capacity can reach(434.4±15)mg/g.Moreover,under the cyclic switching between CO_(2) and nitrogen atmospheres,the GCel/PD material can exhibit good cycling stability.The adsorption capacity is as high as 167.5 mg/g even after three usage cycles.The material's particulate nature facilitates its post-usage separation from the system,exhibits promising potential in protein extraction and separation.

关 键 词：复合水凝胶 CO_(2) 刺激-响应 吸附分离 

分 类 号：O636[理学—高分子化学]