胎儿生长受限患者胎盘组织中VEGFA、HIF-1α表达及临床意义  

Expressions and the clinical significances of the vascular endothelial growth factor A and hypoxia inducible factor-1αin placental tissue of pregnant women with fetal growth restriction

作　　者：王宏艳[1] 徐志文[1] 孙晓东[1] 朱秀民[1] 崔元日[1] WANG Hongyan;XU Zhiwen;SUN Xiaodong;ZHU Xiumin;CUI Yuanri(Daqing Oilfield General Hospital,Daqing,Heilongjiang Province,163001)

机构地区：[1]大庆油田总医院,163001

出　　处：《中国计划生育学杂志》2024年第7期1663-1667,1672,共6页Chinese Journal of Family Planning

摘　　要：目的:探究胎儿生长受限(FGR)患者胎盘组织中血管内皮生长因子A(VEGFA)、缺氧诱导因子(HIF)-1α表达及临床意义。方法:选择2020年5月-2022年9月在本院确诊FGR并住院分娩的孕妇116例(FGR组)、分娩正常孕妇(新生儿正常)116例为对照组临床资料。qRT-PCR检测胎盘组织中VEGFA、HIF-1α的mRNA表达情况,免疫组化法检测VEGFA、HIF-1α的蛋白表达情况。Pearson相关性分析VEGFA与HIF-1α的相关性以及与FGR患者临床资料相关性;多因素logistic回归分析影响FGR发生的因素。结果:FGR组VEGFA蛋白阳性表达率(25.9%)低于对照组(64.7%),HIF-1α蛋白阳性表达率(67.2%)高于对照组(24.1%);FGR组胎盘组织中VEGFA mRNA表达水平(0.75±0.20)低于对照组(1.00±0.23),HIF-1α mRNA表达水平(1.26±0.25)高于对照组(1.01±0.19)(均P<0.05)。FGR患者胎盘组织中VEGFA mRNA与HIF-1α mRNA呈负相关(P<0.05)。FGR患者新生儿出生1 min Apgar评分、胎盘重量、胎盘体积、新生儿体重与VEGFA表达水平呈正相关,与HIF-1α表达水平呈负相关;HIF-1α升高为影响FGR发生的危险因素,VEGFA升高为保护因素(均P<0.05)。结论:FGR患者胎盘组织中VEGFA低表达、HIF-1α高表达,二者影响FGR的发生及新生儿出生质量。Objective:To investigate the expressions and clinical significances of the vascular endothelial growth factor A(VEGFA) and hypoxia inducible factor(HIF)-1α in placental tissue of pregnant women with fetal growth restriction(FGR).Methods:From May 2020 to September 2022,116 pregnant women diagnosed with FGR and hospitalized for delivery in the hospital were selected in study group.Another 116 healthy pregnant women who gave birth in the hospital were included in control group.QRT-PCR was applied to detect the mRNA expressions of VEGFA and HIF-1α in placental tissue of the women in the two groups.Immunohistochemistry was applied to detect the protein expressions of VEGFA and HIF-1α.Pearson correlation was applied to analyze the correlation between the VEGFA level of the women and their HIF-1αlevel and the correlation between the VEGFA and HIF-1αlevels of the women with FGR and their clinical data.Multivariate logistic regression was applied to analyze the factors affecting the FGR occurrence of the women.Results:The positive expression rate(25.9%) of the VEGFA protein of the women in the study group was significantly lower than that(64.7%) of the women in the control group,and the positive expression rate(67.2%) of the HIF-1α protein of the women in the study group was significantly higher than that(24.1%) of the women in the control group.The VEGFA mRNA expression level(0.75±0.20) in the placenta tissue of the women in the study group was significantly lower than that(1.00±0.23) of the women in the control group and the HIF-1αmRNA expression level(1.26±0.25) in the placenta tissue of the women in the study group was significantly higher than that(1.01±19) of the women in the control group(all P<0.05).The VEGFA mRNA level in placental tissues of the women with FGR was negative correlation with their HIF-1α mRNA level(P<0.05).The neonatal Apgar score at 1min after birth,the placental weight,the placental volume and weight of the women with FGR were positively correlated with the expression level of their

关 键 词：胎儿生长受限 胎盘组织 血管内皮生长因子A 缺氧诱导因子-1Α 相关性 影响因素 新生儿 

分 类 号：R714.5[医药卫生—妇产科学]