77例系统性红斑狼疮患者抗核抗体分布及免疫球蛋白和补体水平变化的临床意义  

Clinical significance of anti nuclear antibody distribution and changes in immunoglobulin and complement levels in 77 patients with systemic lupus erythematosus

作　　者：陈雪雯[1] 李宏峰 赵俊芳[1] 宋林[1] 刘平[1] 部楠 Chen Xuewen;Li Hongfeng;Zhao Junfang;Song Lin;Liu Ping;Bu Nan(Laboratory,Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,Tianjin 300120,China)

机构地区：[1]天津市中医药研究院附属医院,天津300120

出　　处：《首都食品与医药》2024年第12期96-98,共3页Capital Food Medicine

摘　　要：目的分析77例首次诊断为系统性红斑狼疮患者抗核抗体分布及免疫球蛋白和补体水平的变化,为临床诊断和治疗提供实验室依据。方法收集2022年在天津市中医药研究院附属医院首次确诊并进行免疫全项检测的系统性红斑狼疮患者77例,采用间接免疫荧光法(IIF)检测抗核抗体(ANA),线性免疫印迹法(LIA)检测抗核抗体谱(ANAs),免疫散射比浊法检测免疫球蛋白和补体。结果77例SLE患者的抗核抗体阳性率为94.81%,抗核抗体谱中抗SS-A(60)抗体、抗SS-A(52)抗体、抗Sm抗体、抗核糖体P蛋白、抗ds-DNA抗体、抗U1-SnRNP抗体和抗SS-B抗体阳性率较高。IgG、IgA、IgM、C3、C4在不同抗核抗体滴度水平下,经方差分析P均>0.05,无统计学差异。ANA阳、ANAs阴组的IgG水平低于ANA、ANAs全阳组,ANA阳、ANAs阴组和ANA与ANAs全阴组的C3、C4水平均高于ANA、ANAs全阳组,差异具有统计学意义(P<0.05)。结论ANAs分布及免疫球蛋白和补体的变化对系统性红斑狼疮的诊疗具有重要意义。Objective To analyze the distribution of anti nuclear antibodies and changes in immunoglobulin and complement levels in 77 patients with systemic lupus erythematosus who were first diagnosed,in order to provide laboratory evidence for clinical diagnosis and treatment.Method 77 patients with systemic lupus erythematosus who were first diagnosed in our hospital and underwent comprehensive immune testing in 2022 were collected.Indirect immunofluorescence(IIF)was used to detect antinuclear antibodies(ANA),linear immunoblotting(LIA)was used to detect antinuclear antibody profiles(ANAs),and immunoassay was used to detect immunoglobulin and complement.Results The positive rate of anti nuclear antibodies in 77 SLE patients was 94.81%,and the positive rates of anti SS-A(60)antibody,anti SS-A(52)antibody,anti Sm antibody,anti ribosomal P protein,anti ds-DNA antibody,anti U1-snRNP antibody,and anti SS-B antibody in the anti nuclear antibody spectrum were relatively high.At different levels of anti nuclear antibody titers,IgG,IgA,IgM,C3,and C4 were analyzed for variance with P>0.05,and there was no statistically significant difference.The IgG levels in the ANAs positive and ANAs negative groups were lower than those in the ANAs and ANAs all positive groups.The C3,and C4 levels in the ANAs positive,ANAs negative groups,and ANAs and ANAs all negative groups were higher than those in the ANAs and ANAs all positive groups,and the difference was statistically significant(P<0.05).Conclusion The distribution of ANAs and changes in immunoglobulin and complement are of great significance for the diagnosis and treatment of systemic lupus erythematosus.

关 键 词：系统性红斑狼疮 抗核抗体 抗核抗体谱 免疫球蛋白 补体 

分 类 号：R593.241[医药卫生—内科学]