基于光子爆发计数的双链DNA抗体均相分析方法研究  

Homogeneous Analysis Method of Anti-dsDNA Antibody Based on Photon Burst Counting Technique

作　　者：万晴 董朝青[1] 任吉存[1] WAN Qing;DONG Chaoqing;REN Jicun(School of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai 200240)

机构地区：[1]上海交通大学化学化工学院,上海200240

出　　处：《分析科学学报》2024年第3期249-254,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21834005,21874090,21675111)。

摘　　要：本文报道了一种基于共振散射光子爆发计数技术的双链DNA抗体(Anti-dsDNA antibody)分析新方法。基于金纳米粒子(Gold Nanoparticles, GNPs)极强的共振光散射性质,以GNPs为探针,通过激光强度、金纳米颗粒表面结合双链DNA数目、探针浓度、反应时间等实验条件的优化,建立了双链DNA抗体的均相免疫定量分析方法。最优实验条件下,该方法的线性范围为1.0×10^(-8)mol/L~1.0×10^(-7)mol/L,检测限为5.2×10^(-9)mol/L。与目前已有的方法相比,该方法具有快速、简单、灵敏度高和特异性好等优点,在临床诊断和生命科学中具有广阔的应用前景。In this paper,a novel homogeneous analysis method for anti-dsDNA antibody was reported based on confocal scattering photon burst counting technique and utilizing the strong resonance light scattering properties of gold nanoparticles(GNPs).The experimental conditions such as laser intensity,the number of the bound double-stranded DNA per GNPs,probe concentration and reaction time were optimized and the quantitative analysis method for anti-dsDNA antibody was established.Under optimal conditions,the linear range of the method is 1.0×10^(-8) mol/L-1.0×10^(-7) mol/L,and the detection limit is 5.2×10^(-9) mol/L.Compared with the existing methods,this method has the advantages of high sensitivity,specificity,good reproducibility,fastness and simplicity,and has a broad application prospect in clinical diagnosis and life science.

关 键 词：光子爆发计数 散射 金纳米粒子 双链DNA抗体 均相分析 

分 类 号：O657[理学—分析化学]