新外来中药紫锥菊对虚热证虚寒证小鼠甲状腺激素和物质能量代谢的影响  

Effects of Echinacea purpurea on Thyroid Hormone and Material and Energy Metabolismin Mice with Deficiency-heat and Deficiency-cold Syndromes

作　　者：郑婷 梁唯灿 吕瑞琳 王禅缔 乐娜 张建军[1] 王林元[1] 陈亮 王淳[1] 郭宝林[3] ZHENG Ting;LIANG Weican;LYU Ruilin;WANG Chandi;YUE Na;ZHANG Jianjun;WANG Linyuan;CHEN Liang;WANG Chun;GUO Baolin(Beijing University of Chinese Medicine,Beijing 100029,China;Amway〔Shanghai〕Innovation&Science,Shanghai 201203,China;Institute of Medicinal Plants,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100005,China)

机构地区：[1]北京中医药大学,北京100029 [2]安利〔上海〕科技发展有限公司,上海201203 [3]中国医学科学院北京协和医学院药用植物研究所,北京100005

出　　处：《世界中医药》2024年第11期1569-1576,共8页World Chinese Medicine

基　　金：国家重点研发计划中医药现代化研究重点专项(2018YFC1706800);国家中医药管理局国际合作司中医药国际合作专项(基地类项目)(0730-236132ZC0054/01~03);国家中医药管理局国际合作司中医药国际合作专项(中心类项目)(0730-236132ZC0052/01~04)。

摘　　要：目的:考察紫锥菊水提物对虚寒、虚热证小鼠内分泌激素、物质能量代谢水平的影响,探讨紫锥菊水提物的药性。方法:将KM雄性小鼠随机分为空白组、虚热模型组、虚热西洋参组、虚热紫锥菊水提物低剂量组、虚热紫锥菊水提物高剂量组、虚寒模型组、虚寒人参组、虚寒紫锥菊水提物低剂量组、虚寒紫锥菊水提物高剂量组;虚热模型组小鼠每天灌胃甲状腺片水溶液(160 mg/kg),虚寒模型组小鼠灌胃氢化可的松溶液(25 mg/kg),1次/d,连续14 d制备虚热、虚寒模型,各给药组灌胃相应药物。检测各组小鼠血清三碘甲状腺原氨酸(T_(3))、甲状腺素(T_(4))、总胆固醇(TC)、三酰甘油(TG)、总蛋白(TP)、白蛋白(ALB)以及血糖(GLU)等指标变化,比色法检测小鼠肝脏乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、Na^(+)-K^(+)-ATP酶活力,采用实时聚合酶链反应法检测小鼠肝组织细胞色素c氧化酶7A2(COX7A2)、琥珀酸脱氢酶复合体D亚基(SDHD)mRNA表达情况。结果:与虚热模型组比较,紫锥菊水提物对虚热小鼠整体状态有一定程度改善,体质量和脾脏指数有所改善(P<0.05),T_(3)、T_(4)水平显著降低(P<0.05,P<0.01),TP、ALB、GLU、LDH的水平显著升高(P<0.01),SDH、Na^(+)-K^(+)-ATP酶、COX7A2、SDHD mRNA的表达水平显著降低(P<0.05,P<0.01),紫锥菊水提物与寒凉性质的西洋参表现出相似的作用;与虚寒模型组比较,紫锥菊水提物对虚寒小鼠整体状态、胸腺指数以及脾脏指数无明显改善,紫锥菊水提物高剂量显著升高SDH、Na^(+)-K^(+)-ATP酶水平(P<0.01),而紫锥菊水提物对虚寒小鼠T_(3)、T_(4)、TP、ALB、TC、TG、GLU、COX7A2 mRNA、SDHD mRNA表达水平的调节不明显,差异无统计学意义(P>0.05),紫锥菊水提物与温热性质的人参作用趋于相反。结论:紫锥菊水提物性凉,可能是紫锥菊寒凉药性的物质基础。Objective:To investigate the effects of water extract of Echinacea purpurea on the endocrine hormones and material and energy metabolism in mice with deficiency-cold and deficiency-heat syndrome and explore the pharmacological characteristics of E.purpurea.Methods:Male KM mice were randomly divided into blank group,deficiency-heat model group,deficiency-heat Panax quinquefolius group,deficiency-heat low-dose E.purpurea extract group,deficiency-heat high-dose E.purpurea extract group,deficiency-cold model group,deficiency-cold P.ginseng group,deficiency-cold low-dose E.purpurea extract group,and deficiency-cold high-dose E.purpurea extract group.Mice in the deficiency-heat model group were administered water solution of thyroid tablets(160 mg/kg)orally daily,while those in the deficiency-cold model group received hydrocortisone solution(25 mg/kg)orally once daily for 14 consecutive days to establish the deficiency-heat and deficiency-cold models,respectively.Each treatment group received corresponding drugs orally.Serum levels of triiodothyronine(T_(3)),thyroxine(T_(4)),total cholesterol(TC),triglycerides(TG),total protein(TP),albumin(ALB),and glucose(GLU)were measured.The activities of lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),and Na^(+)-K^(+)-ATP in the liver were assessed using colorimetric methods.Real-time polymerase chain reaction(PCR)was used to detect mRNA expression of cytochrome c oxidase subunit 7A2(COX7A2)and succinate dehydrogenase complex subunit D(SDHD)in liver tissue.Results:Compared to the deficiency-heat model group,E.purpurea extract improved the overall condition of deficiency-heat mice,with improvements in body weight and spleen index(P<0.05).Levels of T_(3)and T_(4)were significantly reduced(P<0.05,P<0.01),while levels of TP,ALB,GLU,and LDH were significantly increased(P<0.01).Expression levels of SDH,Na^(+)-K^(+)-ATP,COX7A2,and SDHD mRNA were significantly decreased(P<0.05,P<0.01).E.purpurea extract exhibited similar effects to P.quinquefolius with cold-nature properties.Compar

关 键 词：紫锥菊 新外来中药 寒热药性 甲状腺素 物质代谢 能量代谢 虚寒证 虚热证 

分 类 号：R285.5[医药卫生—中药学]