中华跌打丸活血化瘀作用的实验研究  

Effects of Zhonghua Dieda Pills on Promoting Blood Circulation and Resolving Blood Stasis

作　　者：李军梅[1] 郑志远 李鸿海[1] 严灿梅 任常英 王光蕊[1] 刘建勋[1] LI Junmei;ZHENG Zhiyuan;LI Honghai;YAN Canmei;REN Changying;WANG Guangrui;LIU Jianxun(Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;Guangxi Wuzhou Pharmaceutical〔Group〕Co.,Ltd.,Wuzhou 543000,China)

机构地区：[1]中国中医科学院西苑医院中药药理北京市重点实验室,北京100091 [2]广西梧州制药〔集团〕股份有限公司,梧州543000

出　　处：《世界中医药》2024年第11期1614-1618,1624,共6页World Chinese Medicine

基　　金：国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-C-202007);中国中医科学院科技创新工程重大攻关项目(CI2021A04601);广西创新驱动发展专项(桂科AA17202040)。

摘　　要：目的:观察中华跌打丸对大鼠急性血瘀模型的体外血栓形成、血液黏度、血小板聚集率和对小鼠微循环障碍模型耳郭微循环的影响,评价中华跌打丸活血化瘀的作用。方法:通过注射肾上腺素加冰水刺激的方法建立大鼠急性血瘀模型,测定体外血栓形成和血液黏度;按照Born氏比浊法加入诱导剂诱导聚集测定大鼠血小板聚集率;尾静脉注射15%高分子右旋糖酐造成微循环障碍,观察小鼠耳郭各时段微动脉和微静脉管血液流速和血管管径的变化。结果:中华跌打丸可降低血瘀模型大鼠的血液黏度,在切变率150 S^(-1)、60 S^(-1)及5 S^(-1)下的全血黏度均显著降低(P<0.05),明显缩短血瘀模型大鼠血栓长度,减轻血栓湿重和干重(P<0.05,P<0.01);中华跌打丸具有抑制血小板聚集的作用,各剂量均能明显降低ADP诱导的大鼠血小板聚集率(P<0.05,P<0.01);中华跌打丸对高分子右旋糖酐所致小鼠耳郭微循环障碍有明显改善作用,于造模10 min、20 min、30 min时增加血液流速(P<0.05,P<0.01),扩大微动脉、微静脉管径(P<0.05,P<0.01),减轻红细胞聚集(P<0.05,P<0.01)。结论:中华跌打丸可明显改善血液黏度,抑制血小板聚集,改善微循环障碍,有较好的活血化瘀作用。Objective:To observe the effects of Zhonghua Dieda Pills on in vitro thrombosis formation,blood viscosity,platelet aggregation rate in acute blood stasis model rats,and on ear microcirculation in microcirculation disorder model mice to evaluate the blood-activating and stasis-resolving effects of Zhonghua Dieda Pills.Methods:An acute blood stasis model in rats was established by injecting adrenaline and cold water stimulation,and in vitro thrombosis formation and blood viscosity were measured.Platelet aggregation rate in rats was measured using the Born turbidimetric method with an inducer.Microcirculation disorder was induced in mice by tail vein injection of 15%high-molecular dextran,and changes in blood flow velocity and vessel diameter of arterioles and venules in the mouse ear were observed over time.Results:Zhonghua Dieda Pills significantly reduced blood viscosity in blood stasis model rats,with significant reductions in whole blood viscosity at shear rates of 150 S^(-1),60 S^(-1),and 5 S^(-1)(P<0.05).Zhonghua Dieda Pills significantly shortened thrombus length and reduced both wet and dry weights of thrombi in blood stasis model rats(P<0.05,P<0.01).Zhonghua Dieda Pills inhibited platelet aggregation,with all doses significantly reducing ADP-induced platelet aggregation rates in rats(P<0.05,P<0.01).Additionally,Zhonghua Dieda Pills significantly improved microcirculation disorder induced by high-molecular dextran in mice,increased blood flow velocity and expanded arteriole and venule diameters at 10,20,and 30 min post-modeling(P<0.05,P<0.01),and reduced red blood cell aggregation(P<0.05,P<0.01).Conclusion:Zhonghua Dieda Pills can significantly improve blood viscosity,inhibit platelet aggregation,and ameliorate microcirculation disorders,demonstrating a good effect in activating blood circulation and resolving blood stasis.

关 键 词：中华跌打丸 活血化瘀 血瘀模型 微循环障碍 血小板聚集 血液黏度 血栓形成 实验研究 

分 类 号：R285.5[医药卫生—中药学]