补肾运脾方促矮小大鼠生长的效用机制研究  

Effect and Mechanism of Bushen Yunpi Formula in Promoting the Growth of Short Stature Rats

作　　者：张利 赵鋆[1] 金莺[2] 刘建刚 杨楚文 宋勤丽 芦小叶 叶青艳[1] ZHANG Li;ZHAO Jun;JIN Ying;LIU Jiangang;YANG Chuwen;SONG Qinli;LU Xiaoye;YE Qingyan(Pediatric Department,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Intensive Care Rehabilitation Department for Children,Zhejiang Provincial Hospital of Chinese Medicine,Hangzhou 311100,China;Pediatric Department,Shanghai Seventh People′s Hospital,Shanghai 200137,China)

机构地区：[1]上海中医药大学附属曙光医院儿科,上海201203 [2]浙江省中医院儿童重症康复科,杭州311100 [3]上海市第七人民医院儿科,上海200137

出　　处：《世界中医药》2024年第11期1619-1624,共6页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81804141);上海市进一步加快中医药传承创新发展三年行动计划项目[ZY(2021-2023)-0206-02];上海市儿童健康服务能力建设专项(GDEK201704)。

摘　　要：目的:观察补肾运脾方对矮小大鼠生长的影响,探讨其作用机制。方法:将雌性30只、雄性15只大鼠合笼过夜,雌鼠受孕后按随机数字表随机分为低蛋白饲料组与标准饲料组,所有孕鼠分娩后采用标准饲料喂养。仔鼠2周龄去除雌仔鼠,3周龄离乳。标准饲料组孕鼠分娩的新生仔鼠中按随机数字表随机方法选取10只雄鼠仔鼠标记为正常组,选矮小模型仔鼠50只分为模型组、西药组、低剂量组、中剂量组、高剂量组,每组10只。正常组、模型组给予无菌生理盐水灌胃,1 mL/100 g;低、中、高剂量组分别给予补肾运脾方药液0.76 g/mL、1.52 g/mL、3.04 g/mL灌胃,1 mL/100 g;西药组给予重组人生长激素注射液腹部皮下注射(0.84 IU/kg)。每天上午8:00给药,1次/d,连续3周。对比各组身长、体质量、骨密度(BMD)、骨矿盐(BMC)及血清Ⅰ型前胶原羧基端肽(PICP)、Ⅰ型胶原羧基吡啶交联肽(ICTP)、生长激素(GH)、胰岛素样生长因子-1(IGF-1)。结果:各组大鼠治疗后身长、体质量高于治疗前(P<0.05),低剂量组、高剂量组BMD高于模型组(P<0.05),正常组、西药组、中剂量组、高剂量组血清PICP含量低于模型组(P<0.05),中药3组血清PICP含量大于正常组(P<0.05),正常组、中药3组、西药组血清ICTP含量小于模型组(P<0.01),中药3组血清ICTP含量大于正常组(P<0.05);正常组、中药3组、西药组血清GH含量小于模型组(P<0.01),低剂量组、中剂量组血清GH含量大于西药组(P<0.05),低剂量组血清GH含量大于正常组(P<0.05);低剂量组、中剂量组血清IGF-1水平小于西药组(P<0.05)。结论:补肾运脾方可促进矮小大鼠身长、体质量增长,促进骨代谢,增加BMD,其效用机制为通过促进血清GH利用、提高血清IGF-1水平发挥促生长作用。Objective:To observe the effects of the Bushen Yunpi Formula on the growth of short stature rats and explore its mechanisms of action.Methods:Thirty female and fifteen male rats were housed together overnight.After conception,female rats were randomly assigned to a low-protein diet group and a standard diet group based on a random number table.All pregnant rats were fed a standard diet after delivery.Offspring were weaned at 3 weeks old after removing female pups at 2 weeks old.From the offspring of rats fed a standard diet,10 male pups were randomly selected by a random number table and marked as the normal group.Fifty short stature model pups from the offspring of rats fed a standard diet were divided into the model group,Western medicine group,low-dose group,medium-dose group,and high-dose group,with 10 rats per group.The normal group and model group were given sterile physiological saline by gavage at 1 mL/100 g.The low,medium,and high-dose groups were given Bushen Yunpi Formula at 0.76 g/mL,1.52 g/mL,and 3.04 g/mL by gavage at 1 mL/100 g.The Western medicine group was injected subcutaneously in the abdomen with recombinant human growth hormone injection(0.84 IU/kg).Administration was given once daily at 8:00 a.m.for 3 weeks.Comparisons were made of body length,body mass,bone mineral density(BMD),bone mineral content(BMC),serum procollagen typeⅠcarboxy-terminal peptide(PICP),serum typeⅠcollagen cross-linked peptide(ICTP),growth hormone(GH),and insulin-like growth factor-1(IGF-1)among the groups.Results:After treatment,the body length and body mass of rats in each group were higher than those before treatment(P<0.05).The BMD of the low-dose and high-dose groups was higher than that of the model group(P<0.05).The serum PICP levels of the normal group,Western medicine group,medium-dose group,and high-dose group were lower than those of the model group(P<0.05).The serum PICP levels of the three Chinese medicine groups were higher than those of the normal group(P<0.05).The serum ICTP levels of the normal group

关 键 词：补肾运脾方 中药 矮小 大鼠 骨密度 生长激素 胰岛素样生长因子-1 Ⅰ型前胶原羧基端肽 

分 类 号：R272.6[医药卫生—中医儿科学] R711.1[医药卫生—中医学]