血清IL-35、SDF-1在儿童腹型过敏性紫癜诊断中的临床意义  

Clinical significance of serum IL-35 and SDF-1 in diagnosis of abdominal Henoch-Schonlein purpura in children

作　　者：石艳沙 郭玉敏 史凤霞 路冲 陈文华 SHI Yansha;GUO Yumin;SHI Fengxia;LU Chong;CHEN Wenhua(Department of Pediatrics,Handan Municipal Central Hospital,Handan,Hebei 056000,China)

机构地区：[1]河北省邯郸市中心医院儿科,河北邯郸056000

出　　处：《检验医学与临床》2024年第14期2020-2024,共5页Laboratory Medicine and Clinic

基　　金：河北省医学科学研究课题(20190958)。

摘　　要：目的 探讨血清白细胞介素(IL)-35、基质细胞衍生因子-1(SDF-1)在儿童腹型过敏性紫癜(HSP)诊断中的临床意义。方法 选取2018年2月至2020年2月在该院治疗的160例早期HSP患儿作为研究对象,根据是否为腹型HSP分为腹型HSP组(80例)和其他类型HSP组(80例)。根据腹型HSP患儿早期病情程度分为轻度组26例、中度组26例和重度组28例。选取同期80例健康儿童作为对照组。采用酶联免疫吸附试验检测血清IL-35和SDF-1水平。采用Pearson相关分析腹型HSP患儿血清IL-35水平与SDF-1的相关性;采用多因素Logistic回归分析腹型HSP发生的影响因素;采用受试者工作特征(ROC)曲线分析血清IL-35和SDF-1对腹型HSP的诊断价值。结果 腹型HSP组血清IL-35水平明显低于其他类型HSP组、对照组(P<0.05),腹型HSP组血清SDF-1水平明显高于其他类型HSP组、对照组(P<0.05)。随着腹型HSP患儿病情程度的加重,血清IL-35水平明显降低(P<0.05),SDF-1水平明显升高(P<0.05)。腹型HSP患儿血清IL-35水平与SDF-1呈负相关(r=-0.594,P<0.05)。IL-35水平升高是发生腹型HSP的保护因素(P<0.05),SDF-1水平升高是发生腹型HSP的危险因素(P<0.05)。血清IL-35、SDF-1以及二者联合诊断腹型HSP的曲线下面积(AUC)分别为0.783、0.765、0.805,二者联合诊断的AUC优于血清IL-35、SDF-1单独诊断。结论 腹型HSP患儿血清IL-35水平降低,SDF-1水平升高,血清IL-35和SDF-1联合检测可更好地诊断腹型HSP。Objective To explore the clinical significance of serum interleukin-35(IL-35)and stromal cell derived factor-1(SDF-1)in the diagnosis of abdominal Henoch-Schonlein purpura(HSP)in children.Methods A total of 160 children patients with HSP treated in this hospital from February 2018 to February 2020 were selected as the study subjects and divided into the abdominal HSP group(80 cases)and other types of HSP group(80 cases)according to whether or not being abdominal HSP.According to the early disease condition of abdominal HSP,the children patients were divided into the mild group(26 cases),moderate group(26 cases)and severe group(28 cases).Eighty healthy children were selected as the control group.The enzyme linked immunosorbent assay was applied to detect serum IL-35 and SDF-1 levels;the correlation between serum IL-35 and SDF-1 levels in children patients with abdominal HSP was analyzed by Pearson method.The multivariate Logistic regression was adopted to analyze the influencing factors of abdominal HSP occurrence;the receiver operating characteristic(ROC)curve was adopted to analyze the diagnostic value of serum IL-35 and SDF-1 in abdominal HSP.Results The serum IL-35 level in the abdominal HSP group was significantly lower than that in other types of HSP group and control group(P<0.05),while the SDF-1 level in the abdominal HSP group was significantly higher than that in other types of HSP group and control group(P<0.05).With the severity of abdominal HSP increase,serum IL-35 level was decreased significantly(P<0.05),and SDF-1 level was increased significantly(P<0.05).There was a negative correlation between serum IL-35 level and SDF-1 level in children patients with abdominal HSP(r=-0.594,P<0.05).Increased IL-35 level was a protective factor of the occurrence of abdominal HSP(P<0.05),and increased SDF-1 level was a risk factor of the occurrence of abdominal HSP(P<0.05).The areas under the curve(AUC)of serum IL-35,SDF-1 and their combination for diagnosing abdominal HSP were 0.783,0.765 and 0.805 respectively,me

关 键 词：腹型过敏性紫癜 白细胞介素-35 基质细胞衍生因子 诊断 儿童 

分 类 号：R725.5[医药卫生—儿科] R446.11[医药卫生—临床医学]