基于网络药理学探讨益气化痰活血方治疗冠心病的机制  

Mechanism of Yiqi Huatan Huoxue Formula in treating coronary heart disease based on network pharmacology

作　　者：张和针 刘培中[1] 杜鹏冲 刘思 刘擎[1] 李创鹏[1] ZHANG Hezhen;LIU Peizhong;DU Pengchong;LIU Si;LIU Qing;LI Chuangpeng(Department of Cardiology,Zhuhai Branch Hospital,Guangdong Provincial Hospital of Chinese Medicine,Zhuhai,Guangdong 519000)

机构地区：[1]广东省中医院珠海医院心血管内科,广东珠海519000

出　　处：《河北中医》2024年第7期1198-1203,共6页Hebei Journal of Traditional Chinese Medicine

基　　金：珠海市2023年度珠海市社会发展领域科技计划项目(编号:2320004000278)。

摘　　要：目的运用网络药理学研究方法探讨益气化痰活血方治疗冠心病的作用机制。方法在中药系统药理学分析平台(TCMSP)和中药分子机制的生物信息学分析工具(BATMAN-TCM)筛选益气化痰活血方的成分和靶点;并通过相关数据库检索冠心病相关靶点;通过韦恩图得到益气化痰活血方和冠心病的共同靶点,然后利用STRING网站进行化合物-疾病共同靶点蛋白互作分析;利用David数据库进行基因本体(GO)分析和京都基因与基因组百科全书(KEGG)通路富集分析。最后,采用Cytoscape3.82软件构建药物-成分-作用靶点-通路-疾病的网络模型。结果通过筛选得到益气化痰活血方的潜在主要活性成分154个,涉及71个疾病作用靶点,主要活性化合物为柚皮素、槲皮素、川陈皮素、山奈酚等,调节机制与脂质和动脉粥样硬化、白细胞介素17(IL-17)信号通路、细胞凋亡、流体剪切应力和动脉粥样硬化等信号通路有关,从而发挥治疗冠心病的作用。结论益气化痰活血方治疗冠心病是多成分、多靶点、多途径相互作用的结果,为益气化痰活血方的基础或临床研究提供一定的理论依据。Objective To explore the mechanism of Yiqi Huatan Huoxue Formula in the treatment of coronary heart disease by network pharmacology.Methods The components and targets of Yiqi Huatan Huoxue Formula were screened by the Traditional Chinese Medicine Systems Pharmacology(TCMSP)platform and the Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine(BATMAN-TCM).The targets of coronary heart disease were searched in online databases.The common targets of Yiqi Huatan Huoxue Formula and coronary heart disease were obtained by plotting a Venn diagram,followed by an interaction analysis of compound-disease network was conducted by the STRING database.David database was used for gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Finally,Cytoscape 3.82 was used to construct a drug-component-target-pathway-disease network.Results A total of 154 active ingredients and 71 targets of Yiqi Huatan Huoxue Formula were screened.The main active compounds included naringenin,quercetin,nobiletin and kaempferol,which were enriched in the regulation of lipids and atherosclerosis,Interleukin(IL)-17 signaling pathway,cell apoptosis,and fluid shear stress,and thus exerted the role in treating coronary heart disease.Conclusion Yiqi Huatan Huoxue Formula treats coronary heart disease in a multi-component,multi-target and multi-pathway mechanism.Our findings provide a theoretical basis for the basic or clinical research for Yiqi Huatan Huoxue Formula.

关 键 词：网络药理学 益气化痰活血方 冠心病 作用机制 

分 类 号：R541.4[医药卫生—心血管疾病]