外源性硫化氢抑制动脉粥样硬化形成机理探讨  

Mechanism of exogenous hydrogen sulfide inhibiting atherosclerosis

作　　者：宣柳[1] 刘增辉[1] 马筝 王超 XUAN Liu;LIU Zenghui;MA Zheng;WANG Chao(Anhui Academy of Medical Sciences,Anhui Medical College,Hefei 230061,China)

机构地区：[1]安徽省医学科学研究院,安徽医学高等专科学校,合肥230061

出　　处：《中国临床保健杂志》2024年第3期378-381,共4页Chinese Journal of Clinical Healthcare

基　　金：安徽农业大学茶树生物学与资源利用国家重点实验室开发基金项目(SKLTOF 20210119)。

摘　　要：目的探讨硫氢化钠(NaHS,硫化氢供体)对兔动脉粥样硬化的调节作用。方法40只新西兰兔分为正常组、模型组、NaHS组、炔丙基甘氨酸组(PPG,胱硫醚-γ-裂解酶合成酶抑制剂),每组各10只。正常组为普通饲料饲养,其他各组均以高脂饲养(2%胆固醇+10%猪油)。12周后检测血脂含量包括三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C);亚甲蓝法测定血清中硫化氢(H_(2)S)的含量;比色法检测氧化还原指标超氧化物歧化酶(SOD)活性,丙二醛(MDA)含量;HE染色观察主动脉根部组织病理学变化。结果相对于模型组,NaHS组兔血清中血脂变化差异无统计学意义;血清中H_(2)S的含量和SOD活性明显上升,MDA含量明显下降;HE染色显示内膜/中层厚度比值,I+M、内膜/中层面积的比值明显下降(P<0.05)。相对于NaHS组,PPG组中H_(2)S含量和SOD活性明显下降,MDA含量明显上升;HE染色显示内膜/中层厚度比值、I+M、内膜/中层面积的比值明显升高(P<0.05)。结论应用外源性硫化氢可通过改变机体氧化应激状态,抑制动脉粥样硬化的形成。Objective To explore the effect of NaHS(H_(2)S donor)on the pathogenesis of atherosclerosis in rabits.Methods Forty male new Zealand white rabits were divided into normal group,modern group,hyperlipid diet+sodium hydrosulfide(NaHS group),hyperlipid diet+PPG group with 10 rabits in each group.The serum levels of total cholesterol,triglyeride,low density lipoprotein and high density lipoprotein were examined.The methylene blue method was used to determine the content of H_(2)S,the colorimetric method was used to detect the content of SOD and the activity of MDA;Histological changes in aoritic root were studied by H-E staining and histomorphometric analysis.Results Compared with the modern group,the changes of serum lipids in the NaHS group were not significant;the serum of H_(2)S content and SOD activity increased significantly,the MDA content decreased significantly,pathomorphology indicated that intima and media(I+M)became thinned,the thickness ratio of intima/middle and the area of intima to media were decreased(P<0.05).Compared with the NaHS group,the H_(2)S content and SOD activity in the PPG group decreased significantly,and the MDA content increased significantly;HE staining showed the thickness ratio of intima/middle layer,I+M,the area of ratio of intima/middle layer significantly were increased(P<0.05).Conclusions Exogenous hydrogen sulfide can postpone the proceeding of atherosclerosis by changing the oxidative stress state and inhibited the formation of atherosclerosis.

关 键 词：动脉粥样硬化 硫化氢 氧化性应激 模型 动物 

分 类 号：R54[医药卫生—心血管疾病]