原发性尿道炎和非特发性尿道炎男性患者第一尿中的细菌菌群鉴定  

Identification of bacterial flora in the first urine of patients with primary urethritis and male patients with non-idiopathic urethritis

作　　者：丁昱方 陈春燕[1] 夏正君 Ding Yufang;Chen Chunyan;Xia Zhengjun(Department of Laboratory,Dazhou Hospital of Integrated Traditional Chinese and Western Med,Dazhou 635000,China)

机构地区：[1]四川省达州市中西医结合医院检验科,达州635000

出　　处：《国际泌尿系统杂志》2024年第4期669-673,共5页International Journal of Urology and Nephrology

基　　金：四川省中医药管理局科研项目(2021MS274)。

摘　　要：目的运用克隆文库法分析原发性尿道炎和非特发性尿道炎男性患者第一尿中的细菌菌群分布情况。方法收集2019年1月至2020年6月于本院就诊的64份尿道炎男性患者(原发性尿道炎和非特发性尿道炎)第一尿的尿液标本,根据基于16S核糖体RNA基因的克隆文库方法分析细菌菌群分布情况。此外,通过常规PCR方法检测淋病奈瑟菌、沙眼衣原体、生殖支原体、人支原体、解脲支原体或细小脲原体的菌群情况。结果64份尿液标本中,42份通过克隆文库方法成功分析出结果;评估了2781个克隆,并检测到95种细菌系统发育型。微生物系统发育型的克隆文库法检测结果:从6个标本中检测到淋病奈瑟菌,并且是5个标本中的主要细菌种类;从5个标本中检测到生殖器分枝杆菌,并且是3个标本中的优势菌种;从2个标本中检测到解脲支原体;分别在8个和1个标本中分别检测到流感嗜血杆菌和脑膜炎奈瑟菌;在10个样本中检测到阴道加德纳菌。常规PCR中TMA方法从15个标本中检测到沙眼衣原体;在8个样本中检测到淋病奈瑟菌,且是5个标本中的优势菌种,在2个样本中占据100%的菌群。常规PCR中TaqMan法在12个样本中检测到生殖器分枝杆菌,且在2个样本中占据50%以上菌群;在1个样本中检测到淋病奈瑟菌,占比100%。结论克隆文库法可检测菌群中各菌种的占有率,可成为男性尿道炎细菌分析的新方法。Objective To analyze the bacterial flora in the first urine of patients with primary urethritis and non-idiopathic urethritis by using the cloning library method.Methods Sixty-four first urine specimens of male patients with urethritis(primary urethritis and non-idiopathic urethritis)were collected in our hospital from January 2019 to June 2020,based on the 16S ribosomal RNA gene clone library method analyzes the bacterial flora.In addition,conventional PCR methods are used to detect neisseria gonorrhoeae,chlamydia trachomatis,mycoplasma genitalium,mycoplasma hominis,ureaplasma urealyticum or ureaplasma parvum.Results Among the 64 urine specimens,42 were successfully analyzed by the clonal library method,from the specimens,2781 clones were evaluated and 95 bacterial phylogenetic types were detected.Cloning library method:Neisseria gonorrhoeae was detected from 6 specimens,and it was the main bacterial species in 5 specimens.Mycobacterium genitalia was detected from 5 specimens,and it was the advantage of 3 specimens strains.Ureaplasma urealyticum was detected in 2 specimens.Haemophilus influenzae and Neisseria meningitidis were detected in 8 and 1 specimens.Gardnerella vaginalis was detected in 10 specimens.TMA method in conventional PCR detected chlamydia trachomatis from 15 specimens.Neisseria gonorrhoeae was detected in 8 specimens,and it was the dominant strain in 5 specimens,and two samples accounted for 100%of the flora.In conventional PCR,the TaqMan method detected Mycobacterium genitalium in 12 samples and accounted for more than 50%of the flora in 2 samples.Neisseria gonorrhoeae was detected in 1 sample and accounted for 100%.Conclusions The cloning library method can detect the occupancy rate of each species in the flora,and may become a new method for the analysis of male urethritis bacteria.

关 键 词：尿道炎 男(雄)性 尿 细菌 

分 类 号：R695[医药卫生—泌尿科学] R446.5[医药卫生—外科学]