激素依赖及频繁复发肾病综合征患儿外周血B淋巴细胞检测的临床意义  

Clinical significance of peripheral blood B lymphocyte detection in children with hormone dependent and frequent recurrent nephrotic syndrome

作　　者：郭燕 黄春华[1] 张学会 黄宗宣[1] 李娜[1] 陶艳玲[1] Guo Yan;Huang Chunhua;Zhang Xuehui;Huang Zongxuan;Li Na;Tao Yanling(Department of Pediatrics,Affiliated Hospital of Jining Medical College,Jining 272029,China)

机构地区：[1]济宁医学院附属医院儿科,济宁272029

出　　处：《国际泌尿系统杂志》2024年第4期677-681,共5页International Journal of Urology and Nephrology

基　　金：山东省卫健委科研项目(2021HG916)。

摘　　要：目的研究激素依赖及频繁复发肾病综合征(SDNS/FRNS)患儿外周血B淋巴细胞检测的临床意义。方法回顾性分析2022年1月至2023年1月在本院接受初始糖皮质激素(GC)治疗的40例SDNS/FRNS患儿的临床资料,将其设为研究组;选取同期入院接受初始GC治疗的40例非频繁复发肾病综合征患儿设为对照组;比较两组治疗前后的NK细胞绝对数,并对比治疗前后的B淋巴细胞亚群表达水平,同时分析研究组患儿的CD19+B淋巴细胞及其亚群与NK细胞绝对数的关系。结果活动期:研究组的记忆B细胞绝对数、过渡型B细胞绝对数、总CD19+B细胞绝对数高于对照组(均P<0.05);研究组的NK细胞绝对数低于对照组(P<0.05)。缓解期:两组浆细胞绝对数、初始B细胞绝对数、过渡型B细胞绝对数、记忆B细胞绝对数、NK细胞绝对数、总CD19+B细胞绝对数比较,差异均无统计学意义(均P>0.05);两组缓解期总CD19+B细胞绝对数、记忆B细胞绝对数、过渡型B细胞绝对数低于活动期(均P<0.05);两组缓解期与活动期的浆细胞绝对数、初始B细胞绝对数比较,差异均无统计学意义(均P>0.05);两组缓解期的NK细胞绝对数高于活动期(P<0.05)。研究组的NK细胞绝对数与浆母细胞绝对数、初始B细胞绝对数均无相关性(均P>0.05),与记忆B细胞绝对数、过渡型B细胞绝对数、CD19+B细胞绝对数均呈明显负相关(均P<0.05)。结论SDNS/FRNS患儿的外周血B淋巴细胞表型分布与记忆B细胞及过渡型B细胞绝对数存在异常,且过渡型B细胞、记忆B细胞可能均存在清除障碍,而清除过程中NK细胞可能均有参与。Objective To investigate the clinical significance of peripheral blood B lymphocyte detection in children with hormone-dependent and frequently relapsed nephrotic syndrome(SDNS/FRNS).Methods The clinical data of 40 children with SDNS/FRNS who received initial glucocorticoid(GC)treatment in our hospital from January 2022 to January 2023 were retrospectively analyzed,and they were set as the study group.At the same time,40 children with infrequent recurrent nephrotic syndrome who received initial GC treatment were selected as the control group.The absolute number of NK cells before and after treatment was compared between the two groups,and the expression level of B lymphocyte subsets before and after treatment was compared.Meanwhile,the relationship between CD19+B lymphocytes and their subsets and the absolute number of NK cells in the study group was analyzed.Results Active period.The absolute number of memory B cells,transitional B cells and total CD19+B cells in the study group were higher than those in the control group,and the difference was significant(all P<0.05).The absolute number of NK cells in the study group was significantly lower than that in the control group(P<0.05).Remission period.There was no significant difference in the absolute number of plasma cells,initial B cells,transitional B cells,memory B cells,NK cells and total CD19+B cells between the two groups(all P>0.05).The total CD19+B cell absolute number,memory B cell absolute number and transitional B cell absolute number in the remission stage were lower than those in the active stage,the difference was significant(all P<0.05).There was no significant difference in the absolute number of plasma cells and initial B cells between the two groups(all P>0.05).The absolute number of NK cells in the remission stage was higher than that in the active stage,and the difference was significant(P<0.05).In the study group,the absolute number of NK cells was not correlated with the absolute number of plasma blasts,the absolute number of initial B cells(a

关 键 词：肾病综合征 B-淋巴细胞 激素依赖 

分 类 号：R726.9[医药卫生—儿科]