一株猪流行性腹泻病毒变异株的分离、鉴定与全基因组序列分析  

Isolation,identification and whole genome sequence analysis of a porcine epidemic diarrhea virus variant strain

作　　者：刘曦 贾相超 李晨曦 李自力[1,2,3] LIU Xi;JIA Xiang-chao;LI Chen-xi;LI Zi-li(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Preventive Veterinary Medicine in Hubei Province,Wuhan 430070,China;State Key Laboratory of Agricultural Microbiology,Wuhan 430070,China)

机构地区：[1]华中农业大学动物医学院,武汉430070 [2]湖北省预防兽医学重点实验室,武汉430070 [3]农业微生物学国家重点实验室,武汉430070

出　　处：《湖北农业科学》2024年第7期134-140,共7页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(32072845)。

摘　　要：江苏省某猪场疑似发生猪流行性腹泻(Porcine epidemic diarrhea,PED),采集病料后,经RT-PCR检测PEDV呈阳性。病毒感染Vero细胞能够引起明显的细胞病变,间接免疫荧光鉴定结果显示病毒感染细胞后能被抗PEDV N蛋白的特异性单克隆抗体识别。通过全基因组序列分析,鉴定该毒株为PEDV G2b亚型变异株,并将其命名为PEDV JS-B株。PED was suspected to have occurred in a pig farm in Jiangsu Province,and after collection of disease material,PEDV was tested positive by RT-PCR.Virus-infected Vero cells were able to cause significant cytopathic changes,and the immunofluorescence results showed that the virus-infected cells could be recognized by a specific monoclonal antibody against PEDV N protein.Through whole genome sequence analysis,this strain was identified as a PEDV G2b subtype variant and designated PEDV JS-B strain.

关 键 词：猪流行性腹泻病毒 分离鉴定 变异毒株 分段测序 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]