不同产地侧柏叶中无机元素的含量测定及评价  

Determination and evaluation of inorganic elements content in Platycladus orientalis leaves from different regions

作　　者：程芬[1] 杨长花[1] 宋艳丽[1] 杜蓓[1] 胡本祥 CHENG Fen;YANG Chang-hua;SONG Yan-li;DU Bei;HU Ben-xiang(School of Medicine,Shaanxi Institute of International Trade&Commerce,Xi’an 712046,China;School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China)

机构地区：[1]陕西国际商贸学院医药学院,西安712046 [2]陕西中医药大学药学院,西安712046

出　　处：《湖北农业科学》2024年第7期158-164,共7页Hubei Agricultural Sciences

基　　金：陕西省教育厅专项科研计划项目(21JK0511);陕西省重点研发计划项目(2021SF-378);陕西高校青年科技创新团队项目(陕教[2019]90号)。

摘　　要：采用电感耦合等离子体质谱法(ICP-MS)测定不同产地侧柏[Platycladus orientalis(L.)Franco]叶中无机元素含量,并进行方法学验证,采用SPSS软件进行聚类分析和主成分分析,对其特征元素进行评价。结果表明,标准样品中26种元素的线性关系良好,相关系数(R^(2))≥0.9953,各元素的检出限在0.0020~4.2226 ng/mL,精密度、稳定性、重复性符合要求,各元素的平均加样回收率在84.1%~123.6%,相对标准偏差在0.7%~4.5%;28批侧柏叶样品中检测出24种无机元素,含量差异较大,其中,侧柏叶样品K、Ca、Mg的含量均超过1000 mg/kg,Cd、As、Hg、Cu含量均在《中华人民共和国药典:一部》规定的限度范围内,Pb含量为0.602~9.337 mg/kg,有6批侧柏叶样品超出限度;主成分分析显示Fe、Ti、Ca、Ni、Al、Mn、Zn、K为侧柏叶的主要特征性元素;聚类分析显示,地理位置相近的产地能较好地聚为一类,表明侧柏叶中无机元素含量存在明显的地域特征。建立的方法操作简便,分析速度快,灵敏度高,可用于侧柏叶中无机元素含量的测定。The content of inorganic elements in Platycladus orientalis leaves from different regions was determined by inductively cou-pled plasma mass spectrometry(ICP-MS),and methodological validation was carried out.Cluster analysis and principal component analysis were conducted using SPSS software to evaluate the characteristic elements.The results indicated that the linear relationship between the 26 elements in the standard sample was good,with correlation coefficient(R^(2))≥09953,the detection limit of each ele-ment was between 0.0020 and 4.2226 ng/mL,precision,stability,and repeatability met the requirements,and the average sample re-covery rate of each element ranged from 84.1%to 12.3.6%,with a relative standard deviation of 0.7%to 4.5%;24 inorganic elements were detected in 28 batches of Platycladus orientalis leaves samples,with significant differences in content,among them,the content of K,Ca,and Mg in the Platycladus orientalis leaves sample exceeded 1000 mg/kg,and the content of Cd,As,Hg,and Cu were all within the limit range specified in the Pharmacopoeia of the People’s Republic of China:volumeⅠ.The Pb content ranged from 0.602 to 9.337 mg/kg,with 6 batches of Platycladus orientalis leaves exceeding the limit;principal component analysis showed that Fe,Ti,Ca,Ni,Al,Mn,Zn,and K were the main characteristic elements of Platycladus orientalis leaves;cluster analysis showed that pro-duction areas with similar geographical locations could be well clustered together,indicating that there were obvious regional charac-teristics in the content of inorganic elements in Platycladus orientalis leaves.The established method was easy to operate,fast in analy-sis speed,and highly sensitive,and could be used for the determination of inorganic elements content in Platycladus orientalis leaves.

关 键 词：侧柏[Platycladus orientalis(L.)Franco]叶 无机元素 聚类分析 含量测定 评价 

分 类 号：R284.1[医药卫生—中药学]