温胆汤对肥胖代谢相关基因B4 galt7、Sc5d启动子甲基化及表达的影响  

Effect of Wendan Decoction(温胆汤)on Methylation and Expressions of Obesity Metabolism Related Genes B4Galt7 and Sc5d Promoters

作　　者：杨海燕[1] 李锦超 任美玲 吴紫葶 王萍[1] YANG Haiyan;LI Jinchao;REN Meiling;WU Ziting;WANG Ping(Institute of Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi,China;Postgraduate of Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi,China)

机构地区：[1]江西中医药大学中医学院,江西南昌330004 [2]江西中医药大学,江西南昌330004

出　　处：《辽宁中医杂志》2024年第7期177-183,共7页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81960851);江西省自然科学基金项目(20202BABL206132)。

摘　　要：目的 分析中医化痰名方温胆汤对肥胖腹腔脂肪组织代谢相关基因B4 galt7、Sc5d启动子区甲基化及表达的影响。方法 首先采用高脂饲料喂养构建肥胖大鼠模型,通过高、中、低不同剂量温胆汤干预肥胖大鼠,观察药物减重降脂的效应;然后采用Massarray技术检测B4Galt7、Sc5d基因启动子甲基化情况;再分别利用实时荧光定量RT-PCR技术、蛋白质印迹技术检测B4Galt7、Sc5d基因的表达情况,分析启动子区甲基化情况与表达间的关系。结果 (1)温胆汤的干预效果与药物浓度无明显剂量依赖关系,高、中剂量温胆汤对肥胖大鼠具有较好减轻体质量作用;温胆汤对改善肥胖大鼠的血脂中TG、TC、LDL-C水平有积极意义,尤其以高剂量组明显。(2)B4 galt7基因启动子区共检测到有31个位点发生甲基化改变。空白对照组与正常对照组比较平均甲基化程度上升,其中有24个位点甲基化程度上升。在此24个位点中,各药物组基本呈现与空白组相对的调控方向,即甲基化程度较空白对照组下降;Sc5d基因启动子区共检测到有37个位点发生甲基化改变。空白对照组与正常对照组比较平均甲基化程度下降,其中有20个位点甲基化程度下降。在此20个位点中,各药物组基本呈现与空白对照组相对的调控方向,即甲基化程度较空白对照组上升。(3)空白对照组与正常对照组相比B4Galt7 mRNA表达降低,但差异无统计学意义(P>0.05);温胆汤干预后,各药物组表达低于空白对照组,但组间均无统计学差异(P>0.05);空白对照组与正常对照组相比Sc5d mRNA表达增高,差异具有统计学意义(P<0.05);温胆汤干预后,各药物组与空白对照组比较表达降低,其中药物中剂量组降低明显,具有统计学差异(P<0.05)。(4)空白对照组与正常对照组比较,B4Galt7、Sc5d蛋白表达升高明显(P<0.01)。温胆汤干预后,各药物组B4Galt7、Sc5d蛋白表达明显低于空白对照组,且差�Objective To analyze the effect of Wendan Decoction(温胆汤),a famous traditional Chinese medicine formula for resolving phlegm,on the methylation and expressions of B4 galt7 and Sc5d promoter regions in obese abdominal adipose tissue.Methods Firstly,a high-fat diet was used to construct an obese rat model.High,medium and low doses of Wendan Decoction were used to intervene in obese rats and observe the effect of medication on weight loss and lipid reduction.Then,Massray technology was used to detect the methylation of the B4Galt7 and Sc5d gene promoters.Real-time fluorescence quantitative RT-PCR technology and Western blotting technology were used to detect the expressions of B4Galt7 and Sc5d genes,and the relationship between promoter region methylation and expression was analyzed.Results(1)The intervention effect of Wendan Decoction had no obvious dose-response relationship.Wendan Decoction could improve the levels of triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),especially those in the high dose group.(2)A total of 31 methylation changes were detected in the promoter region of the B4Galt7 gene.The average degree of methylation in the blank control group increased compared to those in the normal control group,with 24 sites showing an increase in methylation degree.Among these 24 loci,each drug group showed a relative regulatory direction to the blank group,that is,the degree of methylation decreased compared to that in the blank control group.A total of 37 methylation changes were detected in the promoter region of the Sc5d gene.The average methylation level of the blank control group decreased compared to that of the normal control group,with 20 sites showing a decrease in methylation level.Among these 20 loci,each drug group showed a relative regulatory direction to the blank control group,that is,the degree of methylation increased compared to that of the blank control group.(3)Compared with that in the normal control group,the expression of B4Galt7 mRNA in the blank co

关 键 词：温胆汤 肥胖 代谢 β-1 4-半乳糖基转移酶7 甾醇-c5-去饱和酶 甲基化 

分 类 号：R285[医药卫生—中药学]