钛表面电化学沉积氢氧化镁与氧化镁涂层的抗菌性能与生物活性  

Antibacterial Properties and Biological Activities of Magnesium Hydroxide and Magnesium Oxide Coatings by Electrochemical Deposition on Titanium Surface

作　　者：杜佳恒 范鑫丽 肖东琴[2] 刘刚利 尹一然 贺葵 闫吉元 李忠 谭言飞[4] 段可 DU Jiaheng;FAN Xinli;XIAO Dongqin;LIU Gang Li;Yin Yiran;He Kui;Yan Jiyuan;LI Zhong;TAN Yanfei;DUAN Ke(Department of Bone and Joint Surgery,Affiliated Hospital of Southwest Medical University,Sichuan Engineering Laboratory of Orthopaedic Implant R&D and Application Technology,Luzhou 646000,Sichuan,China;Institute of Tissue Engineering and Stem Cell Research,The Second Clinical College of North Sichuan Medical College,Nanchong Central Hospital,Nanchong 637000,Sichuan,China;Department of Oral and Maxillofacial Surgery,School of Stomatology and Hospital of Stomatology,Qilu Medical College,Shandong University,Shandong Key Laboratory of Oral Tissue Regeneration,Shandong Engineering Laboratory of Oral Biomaterials and Tissue Regeneration,Shandong Clinical Medical Research Center of Oral Diseases,Jinan 250012,China;Sichuan University,Biomaterials Engineering Research Center,Chengdu 610065,China)

机构地区：[1]西南医科大学附属医院骨与关节外科,四川省骨科置入器械研发及应用技术工程实验室,四川泸州646000 [2]川北医学院第二临床学院,南充市中心医院组织工程与干细胞研究所,四川南充637000 [3]山东大学齐鲁医学院口腔医学院,口腔医院口腔颌面外科,山东省口腔组织再生重点实验室,山东省口腔生物材料与组织再生工程实验室,山东省口腔疾病临床医学研究中心,济南250012 [4]四川大学,生物材料工程研究中心,成都610065

出　　处：《硅酸盐学报》2024年第5期1570-1577,共8页Journal of The Chinese Ceramic Society

基　　金：四川省科技计划(2020YFS0455,2022YFS0628);泸州市-西南医科大学合作项目(2020LZXNYDZ08,2020LZXNYD F02);西南医科大学产学研项目(2022CXY03);泸县西南医科大学联合课题(2020LXXNYKD-01)。

摘　　要：骨科植入物相关感染已严重影响手术效果及术后康复,因此需研究具备良好抗菌性能及生物活性的涂层。首先确定电化学沉积氢氧化镁[Mg(OH)_(2)]及氧化镁(MgO)涂层的制备条件,在钛表面沉积不同时间的Mg(OH)_(2)及MgO涂层,评价并比较2种涂层的体外抗菌及生物相容性。各组样品表面均匀分布颗粒样物质[Mg(OH)_(2)及Mg O],颗粒密度与覆盖率随沉积时间增加。Mg(OH)2及MgO涂层对金黄色葡萄球菌及大肠杆菌均具良好抗菌性且抗菌率随沉积时间增强。沉积≤3 h,Mg O抑菌率显著高于Mg(OH)_(2);沉积≥6 h,两者`抑菌率均接近100%。Mg(OH)_(2)及MgO涂层细胞毒性随沉积时间上升,其中沉积0.5、1.0 h的2组样品毒性随培养时间下降,其余3组随培养时间上升。沉积0.5 h的Mg(OH)_(2)及MgO样品与细胞共培养5 d时的细胞存活率为152%、90%;沉积12 h的2组样品与细胞共培养5 d时细胞存活率为2%、6%。研究表明电化学沉积法可成功制备Mg(OH)_(2)涂层,且煅烧后可获得Mg O涂层。沉积1h的Mg(OH)_(2)及Mg O涂层样品具备良好抗菌性能的同时细胞毒性较小。Institute of Tissue Engineering and Stem Cell Research,The Second Clinical College of North Sichuan Medical College,Nanchong Central Hospital;Department of Oral and Maxillofacial Surgery,School of Stomatology and Hospital of Stomatology,Qilu Medical College,Shandong University,Shandong Key Laboratory of Oral Tissue Regeneration,Shandong Engineering Laboratory of Oral Biomaterials and Tissue Regeneration,Shandong Clinical Medical Research Center of Oral Diseases;Sichuan University,Biomaterials Engineering Research Center;Introduction Titanium(Ti)-based implants are one of the most commonly used materials in orthopedic surgery.Although Ti-based implants have good biomechanical properties and biocompatibility,their non-antibacterial shortcomings lead to orthopedic implant-related infections that seriously affect the effect of surgery and postoperative recovery.and serious cases need a second operation and increase the pain and cost of patients.Some studies reported metal or antibiotic antibacterial coatings on Ti-based implants,but they have some disadvantages(i.e.,heavy metal coatings can cause damage to the human body,and excessive use of antibiotic coatings can lead to drug resistance).It is thus necessary to investigate the preparation of coatings with good antibacterial properties and biological activities on the surface of Ti-based implants,so as to provide an effective solution to the problem of bone implant-related infection in clinic.Magnesium(Mg)is a major element in human body.Some studies reported that Mg and its compounds have good antibacterial and biocompatibility.Methods Ti sheets were cleaned and immersed in an electrolyte containing sodium chloride and magnesium chloride(p H=9.5)and applied a DC voltage of 3.0 V for 0-12 h to obtain magnesium hydroxide[Mg(OH)_(2)]coatings.After deposition,Mg(OH)_(2) coatings were calcined in a muffle furnace at a heating rate of 2℃/min at 650℃for 6 h to obtain magnesium oxide(Mg O)coatings.The phases of each group of samples were analyzed by X-ray diffraction(X

关 键 词：骨科植入物 抗菌 电化学沉积 氢氧化镁 氧化镁 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]