基于^(15)N稳定同位素示踪技术的植物组合湿地对氨氮去除途径研究  

Nitrogen Removal Pathways in Plant Combination Wetlands Based on ^(15)N Stable Isotope Tracer Technique

作　　者：徐英华 李希[1] 谢陈 李裕元[1,2] 吴金水 XU Ying-hua;LI Xi;XIE Chen;LI Yu-yuan;WU Jin-shui(Institute of Subtropical Agroecology,Chinese Academy of Sciences/Key Laboratory of Agro-ecological Processes in Subtropical Region,Chinese Academy of Sciences,Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China;College of Biology and Environmental Science,Jishou University,Jishou 416000,China)

机构地区：[1]中国科学院亚热带农业生态研究所/中国科学院亚热带农业生态过程重点实验室,湖南长沙410125 [2]中国科学院大学,北京100049 [3]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《生态与农村环境学报》2024年第7期933-942,共10页Journal of Ecology and Rural Environment

基　　金：国家自然科学基金(42077215);湖南省自然科学基金(2021JJ30765);郴州国家可持续发展议程创新示范区专项(2022sfq02)。

摘　　要：人工湿地是生态修复受污染水体的重要手段,对氮的去除效果显著,但不同植物湿地对氮去除效果差异明显,因此不同类型植物组合后的湿地对氮去除效果及去除途径值得进一步研究。通过室内控制试验,以常见浮水植物绿狐尾藻(Myriophyllum elatinoides)和挺水植物梭鱼草(Pontederia cordata)为研究对象,利用^(15)N稳定同位素示踪技术探究绿狐尾藻、梭鱼草及绿狐尾藻+梭鱼草植物组合对氨氮(NH_(4)^(+)-N)的去除途径。结果表明,绿狐尾藻+梭鱼草植物组合处理相较于单一绿狐尾藻和梭鱼草处理对氮的脱除量分别提高1.8%和3.6%。基于^(15)N质量平衡,绿狐尾藻、梭鱼草和绿狐尾藻+梭鱼草植物组合处理氮去除途径的底泥吸附贡献分别为8.30%、7.96%和6.94%,植物吸收贡献分别为41.76%、35.34%和48.67%,微生物作用去除贡献分别为49.94%、56.71%和44.39%。微生物作用在湿地脱氮过程中占主导地位。相较于单一植物湿地,植物组合湿地对氮的去除效果得到提升,同时促进了植物对水体NH 4+-N的吸收。该研究有助于更好地理解植物组合湿地对氨氮的脱除途径。Constructed wetlands(CWs)are an important method for the ecological remediation of polluted water bodies.Their removal efficiency of nitrogen(N)is remarkable.The N removal efficiency varies across CWs with different plant species.The questions are whether this efficiency is improved in CWs with plant combinations,and how is the removal path worthy of further study.In this study,Myriophyllum elatinoides and Pontederia cordata were used as research objects and examined through controlled laboratory experiments.The pathways for ammonia nitrogen(NH_(4)^(+)-N)removal were investigated using the ^(15)N stable isotope tracer technique in M.elatinoides,P.cordata,and M.elatinoides+P.cordata combination.Compared with single plant,M.elatinoides and P.cordata,N removal amount of M.elatinoides+P.cordata combination was increased by 1.8%and 3.6%,respectively.According to the ^(15)N mass balance,the contributions to N removal by M.elatinoides,P.cordata,and their combination through sediment adsorption were 8.30%,7.96%,and 6.94%,respectively,through plant uptake were 41.76%,35.34%,and 48.67%,respectively,and through microbial action were 49.94%,56.71%,and 44.39%,respectively.Microbial action played a dominant role in N removal process in CWs.Compared with single plant CWs,plant combination CWs could improve N removal efficiency and enhance NH_(4)^(+)-N uptake by plants from water.This study is helpful to better understand the removal pathway of NH_(4)^(+)-N in plant combinations CWs.

关 键 词：人工湿地 植物组合 氨氮 ^(15)N同位素 

分 类 号：X52[环境科学与工程—环境工程] X173