香根草对镉-扑草净复合污染胁迫的生理响应  

Physiological Response of Vetiveria zizanioides to Cadmium-prometryn Composite Pollution Stress

作　　者：杨秀彪 吴珂 欧朝蓉[4] 杨迈 孙仕仙[1,2] YANG Xiu-biao;WU Ke;OU Zhao-rong;YANG Mai;SUN Shi-xian(Yunnan Key Laboratory of Plateau Wetland Conservation,Restoration and Ecological Services,Southwest Forestry University,Kunming 650224,China;National Plateau Wetlands Research Center/School of Ecology and Environment(School of Wetlands),Southwest Forestry University,Kunming 650224,China;Luxi County Water Conservancy Projects Construction Center,Luxi 652400,China;School of Geography and Ecotourism,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学云南省高原湿地保护修复与生态服务重点实验室,云南昆明650224 [2]西南林业大学国家高原湿地研究中心/生态与环境学院(湿地学院),云南昆明650224 [3]泸西县水利工程建设中心,云南泸西652400 [4]西南林业大学地理与生态旅游学院,云南昆明650224

出　　处：《生态与农村环境学报》2024年第7期964-974,共11页Journal of Ecology and Rural Environment

基　　金：云南省基础研究计划重点项目(202201AS070028);国家自然科学基金(42167057);云南省“万人计划”青年拔尖人才专项(990122049)。

摘　　要：以香根草(Vetiveria zizanioides)为供试材料,采用温室水培方法,研究水体中Cd(初始质量浓度为0.5 mg·L^(-1))、扑草净(初始质量浓度为1 mg·L^(-1))单一或复合污染胁迫对香根草鲜重、叶绿素含量、根系活力、抗氧化酶系统的影响。结果表明:与未添加Cd和扑草净处理(CK)相比,香根草总鲜重和鲜重增长率在单一Cd污染胁迫下没有显著差异(P>0.05),但在单一扑草净和Cd-扑草净复合污染胁迫下显著降低(P<0.05),在单一扑草净污染胁迫下分别下降32.72 g和11.66%,在Cd-扑草净复合污染胁迫下分别下降16.85 g和6.69%。香根草叶绿素a(Chla)、叶绿素b(Chlb)和总叶绿素(Chlt)含量在单一扑草净污染胁迫下呈波动下降趋势,在单一Cd和Cd-扑草净复合污染胁迫下呈波动上升趋势。除0和6 d时以外,其他培养时间单一Cd污染、Cd-扑草净复合污染和单一扑草净污染胁迫下香根草根系活力均显著高于CK。随培养时间延长,在Cd和扑草净的单一及复合污染胁迫下香根草根系和茎叶超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)和丙二醛(malondialdehyde,MDA)含量表现出不同的变化趋势。香根草对水体中Cd和扑草净的单一/复合污染胁迫具有较强耐受反应,能通过抗氧化酶系统间的相互调整来应对胁迫。研究结果可为筛选人工湿地植物用以修复重金属、农药的单一/复合污染提供基础理论依据。The response of vetiver grass(Vetiveria zizanioides)to single or co-presence of Cd and prometryn was investigated in hydroponic in the greenhouse.The fresh weight,chlorophyll content,root activity,and antioxidant enzyme system of V.zizanioides were analyzed.The results show that the total fresh weight and fresh weight growth rate of V.zizanioides in single Cd treatment showed no significant difference(P>0.05)compared to the control(without Cd or prometryn).However,single prometryn and Cd-prometryn co-presence significantly decreased(P<0.05)the total fresh weight and growth rate of V.zizanioides.Specifically,single prometryn treatment decreased the total fresh weight and growth rate of V.zizanioides by 32.72 g and 11.66%,respectively.Cd-prometryn co-presence decreased the total fresh weight and growth rate by 16.85 g and 6.69%,respectively.The contents of Chla,Chlb,and Chlt in V.zizanioides were decreased in single prometryn treatment,while increased under single Cd and Cd-prometryn co-presence treatments.V.zizanioides root activity was significantly higher in single Cd or prometryn,and Cd-prometryn co-presence treatments compared to control excepting at 0 d and 6 d.With the extension of cultivation time,the contents of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),and malondialdehyde(MDA)in V.zizanioides roots,stems,and leaves showed different trends under Cd and prometryn single or composite stress.V.zizanioides showed strong tolerance to single/composite pollution stress of Cd and prometryn via adjusting the antioxidant enzyme system.The research results provide theoretical supports for screening artificial wetland plants for efficient remediation of single or composite pollution of heavy metal and pesticide.

关 键 词：香根草 植物修复 镉 扑草净 污染胁迫 

分 类 号：X52[环境科学与工程—环境工程] X592