MicroRNA-let-7a对中耳胆脂瘤细胞侵袭和凋亡的调控作用  

Effect of microRNA-let-7a in invasion and apoptosis in middle ear cholesteatoma keratinocyts

作　　者：陈晓华[1] 秦兆冰[1] CHEN Xiao-hua;QIN Zhao-bing(Department of Otology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第一附属医院耳科,郑州450000

出　　处：《医药论坛杂志》2024年第11期1132-1136,共5页Journal of Medical Forum

基　　金：河南省教育厅科技攻关项目(13A320649)。

摘　　要：目的探讨MicroRNA-let-7a对中耳胆脂瘤细胞的侵袭和凋亡的影响及其作用机制。方法选取2017年8月至2021年3月于郑州大学第一附属医院中耳胆脂瘤患者手术标本65例,提取并培养中耳胆脂瘤细胞进行原代培养,以介导microRNA-let-7a mimics的慢病毒载体分别转染的中耳胆脂瘤角质细胞,分为microRNA-let-7a mimics组、Mock组和NC组,利用实时定量PCR检测胆脂瘤细胞中microRNA-let-7a mimics的表达,观察细胞的生长趋势,并利用FCM测细胞周期,AnnexinV-FITC/PI双染法测定胆脂瘤细胞的凋亡,以及Transwell小室法对胆脂瘤细胞侵袭性进行检测。结果MicroRNA-let-7a mimics在转染microRNA-let-7a mimics的胆脂瘤细胞生长显著低于Mock组和NC组,差异有统计学意义(P<0.05),microRNA-let-7a mimics组和Mock组及NC组的细胞周期分布存在显著性差异,G0/G1期的胆脂瘤细胞比例显著增高,差异有统计学意义(P<0.05);Mock组和NC组的胆脂瘤细胞早期凋亡率显著低于microRNA-let-7a mimics组,差异有统计学意义(P<0.05),两对照组的早期细胞凋亡率差异无统计学意义(P>0.05);microRNA-let-7a mimics组的胆脂瘤角质细胞侵出小室的细胞数显著高于其他两组,差异有统计学意义(P<0.05)。结论MicroRNA-let-7a mimics可以抑制中耳胆脂瘤细胞的生长和侵袭,同时促进中耳胆脂瘤细胞的凋亡。Objective This study aims to investigate the effects of microRNA-7a in molecular mechanisms that control apoptosis and invasion in ear cholesteatoma keratinocyts.Methods Totally 65 cholesteatoma tissues received through operation between Auguest 2017 and March 2021 in the First Affiliated Hospital of Zhengzhou University,and the tissues were processed for cell culture.The keratinocytes of cholesteatoma in middle ear were transfected with lentiviral vectors that mediate microRNA-let-7a mimics,and they were divided into the microRNA-let-7a mimics group,the Mock group,and NC group.Real time-PCR was used to assess the expression levels of microRNA-let-7a.The growth trend of the cells was observed in the three group.FCM,AnnexinV-FITC and Transwell were used to assess the cell cycle,invasion and apoptosis of cholesteatoma keratinocytes,respectively.Results The cell growth of microRNA-let-7a mimics group was lower than that of other two group(P<0.05).The cell cycle distribution of the microRNA-let-7a mimics group,Mock group and NC group showed significant differences,with a marked increase in the proportion of choleateatoma cells in the G0/G1 phase,which was statistically significant.The early apoptosis rate of cholesteatoma keratinocytes in Mock group and NV group was significantly lower than that in microRNA-let-7a mimics group(P<0.05).The cell number of cholesteatoma keratinocyte invasion chamber in microRNA-let-7a mimics group was significantly higher than that in the other two groups(P<0.05).Conclusion The present study revealed that microRNA-let-7a promotes the apoptosis and invasion,inhibits proliferation and growth of cholesteatoma keratinocytes.

关 键 词：MicroRNA-let-7a 中耳胆脂瘤 侵袭 凋亡 

分 类 号：R764.2[医药卫生—耳鼻咽喉科]