三磷酸鸟苷对钼-锌单原子纳米酶的类过氧化物酶活性的影响及其便携式检测  

Effect of Guanosine Triphosphate on Peroxidase-like Activity of Mo-Zn Single-atom Nanozymes and Its Portable Detection

作　　者：宋志敏 张玉 杜衍[1,2] SONG Zhi-Min;ZHANG Yu;DU Yan(State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chenese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]中国科学院长春应用化学研究所,电分析化学国家重点实验室,长春130022 [2]中国科学技术大学应用化学与工程学院,合肥230026

出　　处：《分析化学》2024年第6期799-808,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.22174137)资助。

摘　　要：采用湿化学-热解法制备了具有类过氧化物酶(POD)活性的Mo-Zn单原子纳米酶(Mo-Zn SANs),探究了三磷酸鸟苷(GTP)、三磷酸腺苷(ATP)、三磷酸胞苷(CTP)、三磷酸尿苷(UTP)、二磷酸鸟苷(GDP)、一磷酸鸟苷(GMP)和鸟苷(G)这7种分子对Mo-ZnSANs类POD活性的影响。结果表明,GTP通过静电作用和配位作用与Mo-Zn SANs结合形成新的络合物,由于带负电的磷酸基团的引入,增加了对底物TMB的亲和力,可显著增强Mo-ZnSANs的催化活性,而其它核苷酸如ATP、CTP和UTP等对Mo-ZnSANs的类POD活性几乎没有影响。基于此原理,构建了一种手机辅助信号输出的比色传感器,用于GTP的便携式检测。本方法用于检测GTP时具有良好的灵敏度和选择性,能将GTP与其结构类似物区分开,在最优条件下,检测GTP的线性范围为1~10μmol/L,检出限为0.97μmol/L,满足生物样本的检测需求。Mo-Zn single-atom nanozymes(Mo-Zn SANs)with peroxidase(POD)-like activity was prepared by wet chemical-pyrolysis method.The effects of seven molecules,namely guanosine triphosphate(GTP),adenosine triphosphate(ATP),cytidine triphosphate(CTP),uridine triphosphate(UTP),guanosine diphosphate(GDP),guanosine monophosphate(GMP)and guanosine(G),on the POD-like activity of Mo-Zn SANs were investigated.Among them,GTP combined with Mo-Zn SANs to form a new complex through electrostatic and coordination interactions.The introduction of negatively charged phosphate groups increased the affinity for the substrate 3,3’,5,5’-tetramethyl benzidine(TMB),significantly enhancing the catalytic activity of Mo-Zn SANs.However,other nucleotides such as ATP,CTP and UTP had little effect on the POD-like activity of Mo-Zn SANs.Based on this principle,a colorimetric sensor with smartphone-assisted signal output was constructed for portable detection of GTP.The method exhibited good sensitivity and selectivity for detecting GTP,and could distinguish GTP from its structural analogues.Under optimized conditions,the linear detection range of GTP was 1–10μmol/L and the limit of detection was 0.97μmol/L,meeting the requirements for detection of biological samples.

关 键 词：单原子纳米酶 类过氧化物酶 三磷酸鸟苷 便携式检测 

分 类 号：Q503[生物学—生物化学] O657.3[理学—分析化学]