印迹-解析电喷雾/光电离质谱成像技术研究烤烟叶中化学成分的空间分布  

Study on Spatial Distribution of Chemical Components in Flue Cured Tobacco Leaves by Imprinting Analytical Electrospray Photoionization Mass Spectrometry

作　　者：吕纯纯 姜余婷 胡永华[2] 吴刘天 戚可可 刘成园[1] 潘洋[1] LYU Chun-Chun;JIANG Yu-Ting;HU Yong-Hua;WU Liu-Tian;QI Ke-Ke;LIU Cheng-Yuan;PAN Yang(National Synchrotron Radiation Laboratory,University of Science and Technology of China,Hefei 230029,China;Center of Technology,China Tabacco Anhui Industrial Co.,Ltd.,Hefei 230088,China)

机构地区：[1]中国科学技术大学,国家同步辐射实验室,合肥230029 [2]安徽中烟工业有限责任公司技术中心,合肥230088

出　　处：《分析化学》2024年第6期876-884,共9页Chinese Journal of Analytical Chemistry

基　　金：安徽中烟工业有限责任公司科技计划项目(No.2022340000340020)资助。

摘　　要：利用印迹-解析电喷雾/光电离质谱成像(IM-DESI/PI-MSI)技术对初烤后干烟叶不同部位化学成分的空间分布进行了研究,获得了烟叶中不同化学成分的纵/横向相对含量分布规律。采用印迹方法可将烟叶内部化合物转移至多孔聚四氟乙烯板的表面,实现对烟叶内部物质的检测和可视化分析,并且经解析电喷雾后,光电离质谱成像技术具有对植物样本的无极性歧视、软电离和高电离效率等优点,可同时检测和成像烟叶样本中丰富的化合物。基于高分辨质谱共鉴定出40种化学成分,包括植物碱、氨基酸、糖类、酸、酮和酚等。实验结果表明,烟草的代表性化学成分如植物碱、氨基酸和糖类等,从纵向分析主要分布在近叶尖处,从横向分析主要分布在烟叶左右边缘;并检测出Amadori化合物(1-脱氧-1-L-脯氨酸-D-果糖),发现其含量变化与游离氨基酸(脯氨酸)的含量变化趋势总体一致。此外,对烟草的气候斑点病区域进行了成像研究,发现化合物在气候斑区域呈特异性分布。本方法在烟叶生长应激研究方面具有优势。The imprint desorption electrospray photoionization mass spectrometry was employed to locally image the spatial distribution of chemical components in dried tobacco leaves after initial curing.The relative content distribution of different chemical components was obtained in tobacco leaves.The application of imprinting method could transfer tobacco internal compounds to the surface of porous polytetrafluoroethylene plate,which realized the detection and visual analysis of tobacco internal substances.Besides,the imprint desorption electrospray ionization/post-photoionization(Imprint DESI/PI)mass spectrometry imaging technique had the advantages of nonpolarity discrimination,soft ionization and high ionization efficiency for plant samples,and could simultaneously detect and image rich compounds in tobacco samples.A total of 40 kinds of chemical components including alkaloids,amino acids,sugars,acids,ketones and phenols were identified based on high resolution mass spectrometry.The results showed that the representative chemical components of tobacco,such as alkaloids,amino acids and sugars,were mainly distributed near the leaf tip from the vertical analysis and at the left and right leaf edges from the horizontal analysis.Amadori compound(1-Deoxy-1-L-proline-d-fructose)was detected,and the content of Amadori was found to be consistent with that of free amino acid(proline).In addition,the technique was further used to study the climate spot disease area of tobacco,and it was found that the compounds had specific distribution in the climate spot area,which further proved the superiority of this method in studying the growth stress of tobacco leaves.

关 键 词：烟叶 质谱成像 光电离 印迹 化学成分 

分 类 号：O657.63[理学—分析化学] TS411[理学—化学]